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* '''Description:''' write here <br/><br/>
* '''Description:''' GTPase activating protein, activates [[FlhF]], activates assembly of the flagellar C ring, control of flagellar basal body position <br/><br/>


{| align="right" border="1" cellpadding="2"  
{| align="right" border="1" cellpadding="2"  
|-
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ylxH''
|''flhG ''
|-
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''swrC ''
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylxH ''
|-
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' ||  
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|-
|style="background:#ABCDEF;" align="center"| '''Product''' ||  
|style="background:#ABCDEF;" align="center"| '''Product''' || GTPase activating protein
|-
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|style="background:#ABCDEF;" align="center"|'''Function''' || activation of [[FlhF]]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16410 flhG]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=FlhG FlhG]
|-
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 9.648   
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 9.648   
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 894 bp, 298 aa  
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 894 bp, 298 aa  
|-
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' ||  
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[flhF]]'', ''[[cheB]]''
|-
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ylxH_nucleotide.txt    Gene sequence     (+200bp)  ]'''
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU16410 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU16410 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU16410 DNA_with_flanks]
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ylxH_protein.txt Protein sequence]'''
|-
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:ylxH_context.gif]]
|colspan="2" | '''Genetic context''' <br/> [[Image:ylxH_context.gif]]
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ylxH_1710838_1711734_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:ylxH_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16410]]
|-
|-
|}
|}


__TOC__
__TOC__
<br/><br/><br/><br/>
<br/><br/><br/><br/>
<br/><br/><br/><br/>
<br/><br/>


<br/><br/>
= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[motility and chemotaxis]]}},
{{SubtiWiki category|[[resistance against toxins/ antibiotics]]}}
 
= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[CodY regulon]]}},
{{SubtiWiki regulon|[[DegU regulon]]}},
{{SubtiWiki regulon|[[SigD regulon]]}},
{{SubtiWiki regulon|[[Spo0A regulon]]}}


=The gene=
=The gene=
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=== Basic information ===
=== Basic information ===


* '''Coordinates:'''
* '''Locus tag:''' BSU16410


===Phenotypes of a mutant ===
===Phenotypes of a mutant ===
* susceptible to acriflavine and ethidium bromide, and severe growth inhibition as surfactin concentration increased up to 100ug/ml.


=== Database entries ===
=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16410&redirect=T BSU16410]


* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ylxH.html]
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fla-che.html]


* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10254]
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10254]
Line 53: Line 74:


* '''Catalyzed reaction/ biological activity:'''  
* '''Catalyzed reaction/ biological activity:'''  
** binds [[FlhF]] and stimulates its GTPase activity {{PubMed|22056770}}
** controls the distance between the flagellar bodies {{PubMed|23190039}}


* '''Protein family:'''
* '''Protein family:''' View classification (according to Swiss-Prot)


* '''Paralogous protein(s):'''
* '''Paralogous protein(s):'''
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* '''Kinetic information:'''
* '''Kinetic information:'''


* '''Domains:'''  
* '''[[Domains]]:'''  


* '''Modification:'''
* '''Modification:'''
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* '''Effectors of protein activity:'''
* '''Effectors of protein activity:'''


* '''Interactions:'''
* '''[[SubtInteract|Interactions]]:'''  
** forms homodimers {{PubMed|25733861}}
** [[FlhG]]-[[FlhF]] ([[FlhG]] binds the GTP-bound homodimer of [[FlhF]]) {{PubMed|22056770}}
** [[FlhG]]-([[FliY]]-[[FliM]])-[[FliG]] {{PubMed|25733861}}


* '''Localization:'''
* '''[[Localization]]:''' cytoplasm (homogeneous) and flagellar basal body {{PubMed|25733861,16479537}}


=== Database entries ===
=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16410&redirect=T BSU16410]


* '''Structure:'''
* '''Structure:'''  
** [http://www.rcsb.org/pdb/explore/explore.do?structureId=4RZ3 4RZ3] (from ''Geobacillus thermodenitrificans'') {{PubMed|25733861}}
** [http://www.rcsb.org/pdb/explore.do?structureId=3SYN 3SYN] (complex with [[FlhF]]) {{PubMed|22056770}}


* '''Swiss prot entry:'''
* '''UniProt:'''  
** [http://www.uniprot.org/uniprot/P40742 P40742]
** [http://www.rcsb.org/pdb/explore.do?structureId=3SYN 3SYN] (complex with [[FlhF]]) {{PubMed|22056770}}


* '''KEGG entry:'''
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16410]


* '''E.C. number:'''
* '''E.C. number:'''
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=Expression and regulation=
=Expression and regulation=
* '''Operon:''' part of the [[fla-che operon]]
** ''[[flgB]]-[[flgC]]-[[fliE]]-[[fliF]]-[[fliG]]-[[fliH]]-[[fliI]]-[[fliJ]]-[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|9657996,8157612,15175317}}
** ''[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|20233303}}


* '''Operon:'''  
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ylxH_1710838_1711734_1 ylxH] {{PubMed|22383849}}


* '''Sigma factor:'''  
* '''[[Sigma factor]]:'''  
** ''[[flgB]]'': [[SigA]] {{PubMed|9657996}}, [[SigD]] {{PubMed|9657996}}
** ''[[ylxF]]'': [[SigD]] {{PubMed|20233303}}


* '''Regulation:'''  
* '''Regulation:''' see [[fla-che operon]]


* '''Regulatory mechanism:'''  
* '''Regulatory mechanism:''' see [[fla-che operon]]


* '''Additional information:'''  
* '''Additional information:'''


=Biological materials =
=Biological materials =
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=References=
=References=
==Reviews==
<pubmed> 26195616,, </pubmed>
==Original Publications==
<pubmed>15066026,17850253,14651647, 16479537 9657996,8157612,15175317 22056770 23190039 24386445 25733861</pubmed>


# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
[[Category:Protein-coding genes]]

Latest revision as of 10:54, 24 July 2015

  • Description: GTPase activating protein, activates FlhF, activates assembly of the flagellar C ring, control of flagellar basal body position

Gene name flhG
Synonyms ylxH
Essential no
Product GTPase activating protein
Function activation of FlhF
Gene expression levels in SubtiExpress: flhG
Interactions involving this protein in SubtInteract: FlhG
MW, pI 33 kDa, 9.648
Gene length, protein length 894 bp, 298 aa
Immediate neighbours flhF, cheB
Sequences Protein DNA DNA_with_flanks
Genetic context
This image was kindly provided by SubtiList
Expression at a glance   PubMed















Categories containing this gene/protein

motility and chemotaxis, resistance against toxins/ antibiotics

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU16410

Phenotypes of a mutant

  • susceptible to acriflavine and ethidium bromide, and severe growth inhibition as surfactin concentration increased up to 100ug/ml.

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • binds FlhF and stimulates its GTPase activity PubMed
    • controls the distance between the flagellar bodies PubMed
  • Protein family: View classification (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

<pubmed> 26195616,, </pubmed>

Original Publications

<pubmed>15066026,17850253,14651647, 16479537 9657996,8157612,15175317 22056770 23190039 24386445 25733861</pubmed>

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