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| * '''Description:''' P-loop ATPase <br/><br/>
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''ydiB''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || P-loop ATPase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || unknown
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 4.597
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 474 bp, 158 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[thiL]]'', ''[[ydiC]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12410]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ydiB_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |}
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| __TOC__
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| <br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Coordinates:'''
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| ===Phenotypes of a mutant ===
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| reduced growth rate [http://www.ncbi.nlm.nih.gov/sites/entrez/19246765 PubMed]
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| === Database entries ===
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12199]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' the corresponding ''E. coli'' protein is involved in the control of the activity of the [[Gcp]] homolog [http://www.ncbi.nlm.nih.gov/sites/entrez/19376873 PubMed]
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| * '''Protein family:''' UPF0079 family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:''' K(M) for ATP: 60 myM, V(max) 10 nmol/min [http://www.ncbi.nlm.nih.gov/sites/entrez/19246765 PubMed]
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''Interactions:''' dimerizes and oligomerizes [http://www.ncbi.nlm.nih.gov/sites/entrez/19246765 PubMed], the corresponding ''E. coli'' protein interacts with the protein corresponding to [[YdiC]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19376873 PubMed]
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| * '''Localization:''' cytoplasm with the excepton of the nucleoid region [http://www.ncbi.nlm.nih.gov/sites/entrez/19246765 PubMed]
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| === Database entries ===
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| * '''Structure:'''
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| * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O05515 O05515]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU05910]
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| * '''E.C. number:'''
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| === Additional information===
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| ATPase activity is required for ''in vivo'' function of the protein [http://www.ncbi.nlm.nih.gov/sites/entrez/19246765 PubMed]
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| =Expression and regulation=
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| * '''Operon:'''
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| * '''Sigma factor:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:''' pGP833 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| # Hunt, A., Rawlins, J. P., Thomaides, H. B., and Errington, J. (2006) Functional analysis of 11 putative essential genes in Bacillus subtilis. Microbiology 152, 2895-2907. [http://www.ncbi.nlm.nih.gov/sites/entrez/17005971 PubMed]
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| # Karst JC, Foucher AE, Campbell TL, Di Guilmi AM, Stroebel D, Mangat CS, Brown ED, Jault JM (2009) The ATPase activity of an 'essential' ''Bacillus subtilis'' enzyme, YdiB, is required for its cellular function and is modulated by oligomerization. ''Microbiology'' '''155:''' 944-965. [http://www.ncbi.nlm.nih.gov/sites/entrez/19246765 PubMed]
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| # Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
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