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* '''Description:''' write here <br/><br/>
* '''Description:''' 6-pyruvoyltetrahydropterin synthase, synthesis of the modified ribonucleotide queuosine <br/><br/>


{| align="right" border="1" cellpadding="2"  
{| align="right" border="1" cellpadding="2"  
|-
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ykvK''
|''queD ''
|-
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''queD ''
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ykvK''
|-
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' ||  
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|-
|style="background:#ABCDEF;" align="center"| '''Product''' ||  
|style="background:#ABCDEF;" align="center"| '''Product''' || 6-pyruvoyltetrahydropterin synthase
|-
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|style="background:#ABCDEF;" align="center"|'''Function''' || tRNA modification
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13730 queD]
|-
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 16 kDa, 5.944   
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 16 kDa, 5.944   
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 447 bp, 149 aa  
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 447 bp, 149 aa  
|-
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' ||  
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[queC]]'', ''[[queE]]''
|-
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ykvK_nucleotide.txt    Gene sequence     (+200bp)  ]'''
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU13730 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU13730 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU13730 DNA_with_flanks]
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ykvK_protein.txt Protein sequence]'''
|-
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:ykvK_context.gif]]
|colspan="2" | '''Genetic context''' <br/> [[Image:ykvK_context.gif]]
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=queD_1440100_1440549_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:queD_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13730]]
|-
|-
|}
|}


__TOC__
__TOC__
<br/><br/><br/><br/>
<br/><br/><br/><br/>
<br/><br/><br/><br/>
<br/><br/><br/><br/>
<br/><br/><br/><br/>


<br/><br/>
<br/><br/>
= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[translation]]}}
= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[preQ1 riboswitch]]}}


=The gene=
=The gene=
Line 35: Line 51:
=== Basic information ===
=== Basic information ===


* '''Coordinates:'''
* '''Locus tag:''' BSU13730


===Phenotypes of a mutant ===
===Phenotypes of a mutant ===


=== Database entries ===
=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13730&redirect=T BSU13730]


* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ykvK.html]
* '''DBTBS entry:''' no entry


* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13313]
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13313]


=== Additional information===
=== Additional information===




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* '''Effectors of protein activity:'''
* '''Effectors of protein activity:'''


* '''Interactions:'''
* '''[[SubtInteract|Interactions]]:'''


* '''Localization:'''
* '''[[Localization]]:'''


=== Database entries ===
=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13730&redirect=T BSU13730]


* '''Structure:'''
* '''Structure:'''


* '''Swiss prot entry:'''
* '''UniProt:''' [http://www.uniprot.org/uniprot/O31676 O31676]


* '''KEGG entry:'''
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU13730]


* '''E.C. number:'''
* '''E.C. number:'''
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=Expression and regulation=
=Expression and regulation=


* '''Operon:'''  
* '''Operon:''' ''[[queC]]-[[queD]]-[[queE]]-[[queF]]'' {{PubMed|14660578}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=queD_1440100_1440549_1 queD] {{PubMed|22383849}}


* '''Sigma factor:'''  
* '''Sigma factor:'''  


* '''Regulation:'''  
* '''Regulation:'''  
** repressed in the presence of queuosine ([[preQ1 riboswitch]]) {{PubMed|19285444}}


* '''Regulatory mechanism:'''  
* '''Regulatory mechanism:'''  
** [[preQ1 riboswitch]]: transcriptional antitermination in the absence of queuosine {{PubMed|19285444}}


* '''Additional information:'''  
* '''Additional information:'''


=Biological materials =
=Biological materials =
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=References=
=References=


# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
<pubmed>16411777,18259064,14660578,19285444, 17384645 </pubmed>
 
[[Category:Protein-coding genes]]

Latest revision as of 13:33, 2 April 2014

  • Description: 6-pyruvoyltetrahydropterin synthase, synthesis of the modified ribonucleotide queuosine

Gene name queD
Synonyms ykvK
Essential no
Product 6-pyruvoyltetrahydropterin synthase
Function tRNA modification
Gene expression levels in SubtiExpress: queD
MW, pI 16 kDa, 5.944
Gene length, protein length 447 bp, 149 aa
Immediate neighbours queC, queE
Sequences Protein DNA DNA_with_flanks
Genetic context
This image was kindly provided by SubtiList
Expression at a glance   PubMed
























Categories containing this gene/protein

translation

This gene is a member of the following regulons

preQ1 riboswitch

The gene

Basic information

  • Locus tag: BSU13730

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

<pubmed>16411777,18259064,14660578,19285444, 17384645 </pubmed>

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