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| * '''Description:''' transcription repressor of the pur operon <br/><br/>
| | {{#bank: PurR}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''purR''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yabI ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || transcription repressor of the pur operon
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| |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of purine biosynthesis
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 5.771
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 855 bp, 285 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ispE]]'', ''[[yabJ]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB11823]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:purR_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |}
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| __TOC__
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| <br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Coordinates:'''
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/purR-yabJ.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10110]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Biological activity:''' transcriptional repressor of purine biosynthetic operons
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:''' PRPP acts as inducer, binding results in release of PurR from the operator [http://www.ncbi.nlm.nih.gov/sites/entrez/10919400 PubMed]
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| * '''Interactions:''' [[PurR]]-[[Prs]]
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| * '''Localization:'''
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| === Database entries ===
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| * '''Structure:''' 1P4A (complex with PRPP) [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?uid=25670 NCBI] [http://www.ncbi.nlm.nih.gov/sites/entrez/12837783 PubMed]
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| * '''Swiss prot entry:'''
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00470]
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| * '''E.C. number:'''
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| === Additional information===
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| requires [[YabJ]] for ''in vivo'' activity
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| =Expression and regulation=
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| * '''Operon:''' ''[[purR]]-[[yabJ]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/7638212 PubMed]
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:''' negative autoregulation, induction in the presence of PRPP [http://www.ncbi.nlm.nih.gov/sites/entrez/7638212 PubMed]
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| * '''Regulatory mechanism:''' repression by [[PurR]] [http://www.ncbi.nlm.nih.gov/sites/entrez/7638212 PubMed]
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| # Weng et al. (1995) Identification of the ''Bacillus subtilis pur'' operon repressor. ''Proc. Natl. Acad. Sci. USA'' '''92:''' 7455-7459. [http://www.ncbi.nlm.nih.gov/sites/entrez/7638212 PubMed]
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| # Weng & Zalkin (2000) Mutations in the ''Bacillus subtilis'' purine repressor that perturb PRPP effector function ''in vitro'' and ''in vivo''.''Currr. Microbiol.'' '''41:''' 56-59. [http://www.ncbi.nlm.nih.gov/sites/entrez/10919400 PubMed]
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| # Sinha et al. (2003) The purine repressor of ''Bacillus subtilis'': a novel combination of domains adapted for transcription regulation.''J. Bacteriol.'' '''185:''' 4087-4098. [http://www.ncbi.nlm.nih.gov/sites/entrez/12837783 PubMed]
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| # Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
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