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* '''Description:''' levansucrase <br/><br/>
{{#bank: SacB}}


{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''sacB''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || levansucrase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of sucrose, production of levan
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 52 kDa, 6.175 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1419 bp, 473 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pbpE]]'', ''[[levB]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15450&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:sacB_context.gif]]
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
|-
|}
__TOC__
<br/><br/>
=The gene=
=== Basic information ===
* '''Coordinates:'''
===Phenotypes of a mutant ===
=== Database entries ===
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sacB-yveBA.html]
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10388]
=== Additional information===
=The protein=
=== Basic information/ Evolution ===
* '''Catalyzed reaction/ biological activity:'''
* '''Protein family:'''
* '''Paralogous protein(s):'''
=== Extended information on the protein ===
* '''Kinetic information:'''
* '''Domains:'''
* '''Modification:'''
* '''Cofactor(s):'''
* '''Effectors of protein activity:'''
* '''Interactions:'''
* '''Localization:''' extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
=== Database entries ===
* '''Structure:'''
* '''Swiss prot entry:'''
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU34450]
* '''E.C. number:'''
=== Additional information===
=Expression and regulation=
* '''Operon:'''
* '''[[Sigma factor]]:''' [[SigA]]
* '''Regulation:''' induction by sucrose (at high concentration)
* '''Regulatory mechanism:''' induction by [[SacY]]-dependent RNA switch (transcriptional antitermination), in addition activated by [[DegU]]-P
* '''Additional information:'''
=Biological materials =
* '''Mutant:'''
* '''Expression vector:'''
       
* '''lacZ fusion:'''
* '''GFP fusion:'''
* '''two-hybrid system:'''
* '''Antibody:'''
=Labs working on this gene/protein=
=Your additional remarks=
=References=
# Voigt et al. (2009) Cell physiology and protein secretion of ''Bacillus licheniformis'' compared to ''Bacillus subtilis''. ''J Mol Microbiol Biotechnol.'' '''16:''' 53-68 [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
# Crutz AM, Steinmetz M, Aymerich S, Richter R, Le Coq D (1990) Induction of levansucrase in Bacillus subtilis: an antitermination mechanism negatively controlled by the phosphotransferase system. J Bacteriol 172:1043-1050. [http://www.ncbi.nlm.nih.gov/sites/entrez/2105292 PubMed]
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Latest revision as of 09:26, 11 August 2015