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New page: * '''Description:''' write here <br/><br/> {| align="right" border="1" cellpadding="2" |- |style="background:#ABCDEF;" align="center"|'''Gene name''' |''ywtF'' |- |style="background:#ABC...
 
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* '''Description:''' write here <br/><br/>
* '''Description:''' phosphotransferase, attaches teichoic acid, teichuronic acid, or acidic capsular polysaccharide to cell wall peptidoglycan via phosphodiester linkage to N-acteylmuramic acid <br/><br/>


{| align="right" border="1" cellpadding="2"  
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' ||  
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|-
|style="background:#ABCDEF;" align="center"| '''Product''' ||  
|style="background:#ABCDEF;" align="center"| '''Product''' || phosphotransferase, responsible for attachment of anionic polymers to peptidoglycan
|-
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|style="background:#ABCDEF;" align="center"|'''Function''' || transfer of anionic cell wall polymers from lipid-linked precursors to peptidoglycan
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35840 tagT]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=TagT TagT]
|-
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 9.554   
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 9.554   
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 966 bp, 322 aa  
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 966 bp, 322 aa  
|-
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' ||  
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywtG]]'', ''[[ywtE]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU35840 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU35840 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU35840 DNA_with_flanks]
|-
|-
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ywtF_nucleotide.txt    Gene sequence      (+200bp)  ]'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ywtF_protein.txt Protein sequence]'''
|-
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:ywtF_context.gif]]
|colspan="2" | '''Genetic context''' <br/> [[Image:ywtF_context.gif]]
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywtF_3694239_3695207_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:tagT_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35840]]
|-
|-
|}
|}


__TOC__
__TOC__
<br/><br/><br/><br/>
<br/><br/><br/><br/>
<br/><br/><br/><br/>
<br/><br/>
= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[cell wall synthesis]]}},
{{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}},
{{SubtiWiki category|[[membrane proteins]]}}


<br/><br/>
= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[SigM regulon]]}}


=The gene=
=The gene=
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=== Basic information ===
=== Basic information ===


* '''Coordinates:'''
* '''Locus tag:''' BSU35840


===Phenotypes of a mutant ===
===Phenotypes of a mutant ===
* a ''[[tagT]]'' mutant has no phenoype, the triple ''[[tagT]] [[tagU]] [[tagV]]'' mutant is unable to grow under normal conditions {{PubMed|21964069}}


=== Database entries ===
=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35840&redirect=T BSU35840]


* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywtF.html]
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywtF.html]
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* '''Catalyzed reaction/ biological activity:'''  
* '''Catalyzed reaction/ biological activity:'''  


* '''Protein family:'''
* '''Protein family:''' LytR-Cps2a-Psr family  {{PubMed|19099556}}


* '''Paralogous protein(s):'''
* '''Paralogous protein(s):''' [[TagU]], [[TagV]]


=== Extended information on the protein ===
=== Extended information on the protein ===
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* '''Modification:'''
* '''Modification:'''


* '''Cofactor(s):'''
* '''Cofactor(s):''' Mg(2+) {{PubMed|21964069}}


* '''Effectors of protein activity:'''
* '''Effectors of protein activity:'''


* '''Interactions:'''
* '''[[SubtInteract|Interactions]]:'''  
** [[MreB]]-[[TagT]] {{PubMed|21964069}}


* '''Localization:'''
* '''[[Localization]]:'''
** cytoplasmic membrane (with extracytoplasmic catalaytic domain) {{PubMed|21964069}}
** extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]


=== Database entries ===
=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35840&redirect=T BSU35840]


* '''Structure:'''
* '''Structure:'''  
** [http://www.pdb.org/pdb/explore/explore.do?structureId=3MEJ 3MEJ]
** [http://www.rcsb.org/pdb/explore/explore.do?structureId=4DE9 4DE9] {{PubMed|22432711}}


* '''Swiss prot entry:'''
* '''UniProt:''' [http://www.uniprot.org/uniprot/Q7WY78 Q7WY78]


* '''KEGG entry:'''
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU35840]


* '''E.C. number:'''
* '''E.C. number:'''
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=Expression and regulation=
=Expression and regulation=


* '''Operon:'''  
* '''Operon:''' ''ywtF'' {{PubMed|9353933}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywtF_3694239_3695207_1 tagT] {{PubMed|22383849}}


* '''Sigma factor:'''  
* '''[[Sigma factor]]:''' [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 Pubmed]


* '''Regulation:'''  
* '''Regulation:'''  
** expressed at high salt concentrations ([[SigM]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 Pubmed]


* '''Regulatory mechanism:'''  
* '''Regulatory mechanism:'''  


* '''Additional information:'''  
* '''Additional information:'''
** number of protein molecules per cell (minimal medium with glucose and ammonium): 105 {{PubMed|24696501}}
** number of protein molecules per cell (complex medium with amino acids, without glucose): 258 {{PubMed|24696501}}


=Biological materials =
=Biological materials =
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=References=
=References=
==Reviews==
<pubmed> 24024634 </pubmed>
==Original publications==
<pubmed>18957862, 9353933 18179421 19099556 21964069 22432711</pubmed>


# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
[[Category:Protein-coding genes]]

Latest revision as of 11:45, 19 June 2014

  • Description: phosphotransferase, attaches teichoic acid, teichuronic acid, or acidic capsular polysaccharide to cell wall peptidoglycan via phosphodiester linkage to N-acteylmuramic acid

Gene name ywtF
Synonyms
Essential no
Product phosphotransferase, responsible for attachment of anionic polymers to peptidoglycan
Function transfer of anionic cell wall polymers from lipid-linked precursors to peptidoglycan
Gene expression levels in SubtiExpress: tagT
Interactions involving this protein in SubtInteract: TagT
MW, pI 35 kDa, 9.554
Gene length, protein length 966 bp, 322 aa
Immediate neighbours ywtG, ywtE
Sequences Protein DNA DNA_with_flanks
Genetic context
This image was kindly provided by SubtiList
Expression at a glance   PubMed















Categories containing this gene/protein

cell wall synthesis, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

  • Locus tag: BSU35840

Phenotypes of a mutant

  • a tagT mutant has no phenoype, the triple tagT tagU tagV mutant is unable to grow under normal conditions PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: LytR-Cps2a-Psr family PubMed

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Effectors of protein activity:
  • Localization:
    • cytoplasmic membrane (with extracytoplasmic catalaytic domain) PubMed
    • extracellular (signal peptide) PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed at high salt concentrations (SigM) Pubmed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 105 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 258 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

<pubmed> 24024634 </pubmed>

Original publications

<pubmed>18957862, 9353933 18179421 19099556 21964069 22432711</pubmed>

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