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* '''Description:''' low affinity potassium  transporter KtrCD, peripheric membrane component <br/><br/>
{{#bank: ktrC}}
 
* '''Description:''' low affinity potassium  transporter [[KtrC]]-[[KtrD]], peripheric membrane component <br/><br/>


{| align="right" border="1" cellpadding="2"  
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
|-
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || low affinity potassium  transporter KtrCD, peripheric membrane component (proton symport)
|style="background:#ABCDEF;" align="center"| '''Product''' || low affinity potassium  transporter [[KtrC]]-[[KtrD]], <br/>peripheric membrane component (proton symport)
|-
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || potassium uptake  
|style="background:#ABCDEF;" align="center"|'''Function''' || potassium uptake  
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14510 ktrC]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=KtrC KtrC]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://cellpublisher.gobics.de/view/GqbblRJkBH Metal ion homeostasis], [http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=ktrC ktrC]'''
|-
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 24 kDa, 5.63   
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 24 kDa, 5.63   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykqA]]'', ''[[adeC]]''
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykqA]]'', ''[[adeC]]''
|-
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ykqB_nucleotide.txt    Gene sequence     (+200bp)  ]'''
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU14510 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU14510 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU14510 DNA_with_flanks]
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ykqB_protein.txt Protein sequence]'''
|-
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:ykqB_context.gif]]
|colspan="2" | '''Genetic context''' <br/> [[Image:ykqB_context.gif]]
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ktrC_1520531_1521196_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:ktrC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14510]]
|-
|-
|}
|}


__TOC__
__TOC__
<br/><br/><br/><br/>
<br/><br/><br/><br/>
<br/><br/><br/><br/>
<br/><br/>
= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[transporters/ other]]}},
{{SubtiWiki category|[[metal ion homeostasis (K, Na, Ca, Mg)]]}},
{{SubtiWiki category|[[coping with hyper-osmotic stress]]}},
{{SubtiWiki category|[[membrane proteins]]}}
= This gene is a member of the following [[regulons]] =


<br/><br/>


=The gene=
=The gene=
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=== Basic information ===
=== Basic information ===


* '''Coordinates:'''
* '''Locus tag:''' BSU14510


===Phenotypes of a mutant ===
===Phenotypes of a mutant ===


=== Database entries ===
=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU14510&redirect=T BSU14510]


* '''DBTBS entry:''' no entry
* '''DBTBS entry:''' no entry
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=== Additional information===
=== Additional information===


=The protein=
=The protein=
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* '''Catalyzed reaction/ biological activity:'''  
* '''Catalyzed reaction/ biological activity:'''  


* '''Protein family:'''
* '''Protein family:'''  


* '''Paralogous protein(s):''' [[KtrA]]
* '''Paralogous protein(s):''' [[KtrA]]
Line 63: Line 83:
* '''Kinetic information:'''
* '''Kinetic information:'''


* '''Domains:'''  
* '''[[Domains]]:'''  
** contains a [[RCK_N domain]] at the N-terminus (according to UniProt, [http://www.uniprot.org/uniprot/?query=domain:%22RCK+N-terminal+domain*%22])
** contains a c-di-AMP-binding [[RCK_C domain]] at the C-terminus (according to UniProt, [http://www.uniprot.org/uniprot/?query=domain:%22RCK+C-terminal+domain*%22]) {{PubMed|23671116}}


* '''Modification:'''
* '''Modification:'''


* '''Cofactor(s):'''
* '''[[Cofactors]]:'''


* '''Effectors of protein activity:'''
* '''Effectors of protein activity:'''
** the protein binds c-di-AMP {{PubMed|23671116}}


* '''Interactions:''' [[KtrC]]-[[KtrD]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12562800 PubMed]
* '''[[SubtInteract|Interactions]]:'''
** [[KtrC]]-[[KtrD]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12562800 PubMed]


* '''Localization:''' peripheral membrane protein [http://www.ncbi.nlm.nih.gov/sites/entrez/12562800 PubMed]
* '''[[Localization]]:''' peripheral membrane protein [http://www.ncbi.nlm.nih.gov/sites/entrez/12562800 PubMed]


=== Database entries ===
=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU14510&redirect=T BSU14510]


* '''Structure:'''
* '''Structure:'''


* '''Swiss prot entry:'''
* '''UniProt:''' [http://www.uniprot.org/uniprot/P39760 P39760]


* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU14510]
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU14510]


* '''E.C. number:'''
* '''E.C. number:'''
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=Expression and regulation=
=Expression and regulation=


* '''Operon:''' ''ktrC'' [http://www.ncbi.nlm.nih.gov/sites/entrez/12562800 PubMed]
* '''Operon:''' ''ktrC'' {{PubMed|12562800}}


* '''[[Sigma factor]]:'''  
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ktrC_1520531_1521196_1 ktrC] {{PubMed|22383849}}


* '''Regulation:'''  
* '''Sigma factor:'''
 
* '''Regulation:''' constitutively expressed {{PubMed|12562800}}


* '''Regulatory mechanism:'''  
* '''Regulatory mechanism:'''  


* '''Additional information:'''
* '''Additional information:'''
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 576 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 453 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 601 {{PubMed|21395229}}


=Biological materials =
=Biological materials =
* '''Mutant:'''
* '''Mutant:'''
** GHB6 (''ktrC''::''spec''), {{PubMed|12562800}} (available in [[Erhard Bremer]]'s and [[Jörg Stülke]]'s) labs, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A955&Search=1A955 BGSC] as 1A955
** GP2079 (''[[ktrC]]''::''tet''), available in [[Jörg Stülke]]'s lab
** GP2083 (D(''[[ktrA]]-[[ktrB]]'')::''aphA3'' D''[[ktrC]]''::''tet''), available in [[Jörg Stülke]]'s lab


* '''Expression vector:'''
* '''Expression vector:'''
Line 120: Line 152:


=References=
=References=
==Reviews==
<pubmed>25869574 </pubmed>
==Original publications==
<pubmed>12562800,23671116, </pubmed>


# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
[[Category:Protein-coding genes]]

Latest revision as of 14:33, 25 November 2016

ktrC

  • Description: low affinity potassium transporter KtrC-KtrD, peripheric membrane component

Gene name ktrC
Synonyms ylxV, yzaC, ykqB
Essential no
Product low affinity potassium transporter KtrC-KtrD,
peripheric membrane component (proton symport)
Function potassium uptake
Gene expression levels in SubtiExpress: ktrC
Interactions involving this protein in SubtInteract: KtrC
Metabolic function and regulation of this protein in SubtiPathways:
Metal ion homeostasis, ktrC
MW, pI 24 kDa, 5.63
Gene length, protein length 663 bp, 221 aa
Immediate neighbours ykqA, adeC
Sequences Protein DNA DNA_with_flanks
Genetic context
This image was kindly provided by SubtiList
Expression at a glance   PubMed















Categories containing this gene/protein

transporters/ other, metal ion homeostasis (K, Na, Ca, Mg), coping with hyper-osmotic stress, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU14510

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): KtrA

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:
    • the protein binds c-di-AMP PubMed

Database entries

  • Structure:
  • KEGG entry: [4]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation: constitutively expressed PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 576 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 453 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 601 PubMed

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Erhard Bremer, University of Marburg, Germany homepage

Your additional remarks

References

Reviews

<pubmed>25869574 </pubmed>

Original publications

<pubmed>12562800,23671116, </pubmed>

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