|
|
| (42 intermediate revisions by 7 users not shown) |
| Line 1: |
Line 1: |
| * '''Description:''' DNA polymerase X, involved in DNA repair <br/><br/>
| | {{#bank: PolX}} |
|
| |
|
| {| align="right" border="1" cellpadding="2"
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''polX''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yshC ''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' ||
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || DNA polymerase X
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || involved in base excision repair
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 63 kDa, 5.308
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1710 bp, 570 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' ||
| |
| |-
| |
| |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/yshC_nucleotide.txt Gene sequence (+200bp) ]'''
| |
| |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/yshC_protein.txt Protein sequence]'''
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:yshC_context.gif]]
| |
| |-
| |
| |}
| |
|
| |
| __TOC__
| |
|
| |
| <br/><br/>
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Coordinates:'''
| |
|
| |
| ===Phenotypes of a mutant ===
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yshC.html]
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12327]
| |
|
| |
| === Additional information===
| |
|
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:''' template-dependent DNA polymerase, fills single nucleotide gaps [http://www.ncbi.nlm.nih.gov/sites/entrez/18938175 PubMed], has intrinsic 3'-5' exonuclease activity for resecting unannealed 3'-termini in gapped DNA substrates [http://www.ncbi.nlm.nih.gov/sites/entrez/18776221 PubMed]
| |
|
| |
| * '''Protein family:''' DNA polymerase X family
| |
|
| |
| * '''Paralogous protein(s):'''
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''Domains:'''
| |
|
| |
| * '''Modification:'''
| |
|
| |
| * '''Cofactor(s):'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''Interactions:''' monomeric [http://www.ncbi.nlm.nih.gov/sites/entrez/18938175 PubMed]
| |
|
| |
| * '''Localization:'''
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''Structure:'''
| |
|
| |
| * '''Swiss prot entry:'''
| |
|
| |
| * '''KEGG entry:'''
| |
|
| |
| * '''E.C. number:'''
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
|
| |
| * '''Operon:'''
| |
|
| |
| * '''Sigma factor:'''
| |
|
| |
| * '''Regulation:'''
| |
|
| |
| * '''Regulatory mechanism:'''
| |
|
| |
| * '''Additional information:'''
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:'''
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| [[Margarita Salas]], Madrid, Spain [http://www.cbm.uam.es/mkfactory.esdomain/webs/CBMSO/plt_LineasInvestigacion.aspx?IdObjeto=28 link]
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
|
| |
| # Banos et al. (2008) Characterization of a Bacillus subtilis 64 kDa DNA polymerase X potentially involved in DNA repair. ''J. Mol. Biol.'' '''384:''' 1019-1028. [http://www.ncbi.nlm.nih.gov/sites/entrez/18938175 PubMed]
| |
| # Banos et al. (2008) Editing of misaligned 3'-termini by an intrinsic 3'-5' exonuclease activity residing in the PHP domain of a family X DNA polymerase. ''Nucl. Acids Res.'' '''36:''' 5736-5749. [http://www.ncbi.nlm.nih.gov/sites/entrez/18776221 PubMed]
| |
