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| * '''Description:''' transcriptional antiterminator for the sacP-sacA operon <br/><br/>
| | {{#bank: SacT}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''sacT''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipa-47d ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional antiterminator
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| |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of sucrose utilization
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 5.587
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 828 bp, 276 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywcJ]]'', ''[[ywcI]]''
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| |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/sacT_nucleotide.txt Gene sequence (+200bp) ]'''
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| |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/sacT_protein.txt Protein sequence]'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:sacT_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |}
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| __TOC__
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| <br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Coordinates:'''
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sacT.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10593]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' binding to the mRNA of the ''[[sacP]]-[[sacA]]'' operon, causes transcription antitermination (in presence of sucrose and absence of glucose)
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| * '''Protein family:''' BglG family of antiterminators
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| * '''Paralogous protein(s):''' [[LicT]], [[SacY]], [[GlcT]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''Interactions:'''
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| * '''Localization:'''
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| === Database entries ===
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| * '''Structure:'''
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| * '''Swiss prot entry:'''
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU38070]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| * '''Sigma factor:'''
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| * '''Regulation:''' repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| # Mäder et al. (2002) Transcriptome and Proteome Analysis of ''Bacillus subtilis'' Gene Expression Modulated by Amino Acid Availability. ''J. Bacteriol'' '''184:''' 1844288-4295 [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]
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| # Débarbouillé, M., Arnaud, M., Fouet, A., Klier, A., and Rapoport, G. (1990) The sacT gene regulating the ''sacPA'' operon in ''Bacillus subtilis'' shares strong homology with transcriptional antiterminators. J Bacteriol 172: 3966-3973. [http://www.ncbi.nlm.nih.gov/sites/entrez/2163394 PubMed]
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| # Arnaud, M., Vary, P., Zagorec, M., Klier, A., Débarbouillé, M., Postma, P., and Rapoport, G. (1992) Regulation of the ''sacPA'' operon of ''Bacillus subtilis'': identification of phosphotransferase system components involved in SacT activity. J Bacteriol 174: 3161-3170. [http://www.ncbi.nlm.nih.gov/sites/entrez/1577686 PubMed]
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| # Arnaud, M., Débarbouillé, M., Rapoport, G., Saier, M. H., and Reizer, J. (1996) In vitro reconstitution of transcriptional antitermination by the SacT and SacY proteins of ''Bacillus subtilis''. J Biol Chem 271: 18966-18972. [http://www.ncbi.nlm.nih.gov/sites/entrez/8702561 PubMed]
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| # Aymerich, S. and Steinmetz, M. (1992) Specificity determinants and structural features in the RNA target of the bacterial antiterminator proteins of the BglG/SacY family. Proc. Natl. Acad. Sci. USA 89, 10410-10414. [http://www.ncbi.nlm.nih.gov/sites/entrez/1279678 PubMed]
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| # Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
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