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OpuAC: Difference between revisions

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* '''Description:''' glycine betaine ABC transporter (binding protein) <br/><br/>
{{#bank: OpuAC}}


{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''opuAC''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || glycine betaine ABC transporter (binding protein)
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || compatible solute transport
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 8.015 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 879 bp, 293 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[opuAB]]'', ''[[amhX]]''
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/opuAC_nucleotide.txt    Gene sequence      (+200bp)  ]'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/opuAC_protein.txt Protein sequence]'''
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:opuAC_context.gif]]
|-
|}
__TOC__
<br/><br/>
=The gene=
=== Basic information ===
* '''Coordinates:'''
===Phenotypes of a mutant ===
=== Database entries ===
* '''DBTBS entry:''' no entry
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11372]
=== Additional information===
=The protein=
=== Basic information/ Evolution ===
* '''Catalyzed reaction/ biological activity:'''
* '''Protein family:'''
* '''Paralogous protein(s):'''
=== Extended information on the protein ===
* '''Kinetic information:'''
* '''Domains:'''
* '''Modification:'''
* '''Cofactor(s):'''
* '''Effectors of protein activity:'''
* '''Interactions:'''
* '''Localization:'''
=== Database entries ===
* '''Structure:'''
* '''Swiss prot entry:'''
* '''KEGG entry:'''
* '''E.C. number:'''
=== Additional information===
=Expression and regulation=
* '''Operon:'''
* '''Sigma factor:'''
* '''Regulation:'''
* '''Regulatory mechanism:'''
* '''Additional information:'''
=Biological materials =
* '''Mutant:'''
* '''Expression vector:'''
       
* '''lacZ fusion:'''
* '''GFP fusion:'''
* '''two-hybrid system:'''
* '''Antibody:'''
=Labs working on this gene/protein=
=Your additional remarks=
=References=
# Smits SH, Höing M, Lecher J, Jebbar M, Schmitt L, Bremer E. (2008) The compatible-solute-binding protein OpuAC from Bacillus subtilis: ligand binding, site-directed mutagenesis, and crystallographic studies. ''J Bacteriol. '' '''Aug;190(16):''' 5663-71. [http://www.ncbi.nlm.nih.gov/sites/entrez/18567662 PubMed]
# Kempf B, Bremer E. (1995) OpuA, an osmotically regulated binding protein-dependent transport system for the osmoprotectant glycine betaine in Bacillus subtilis. ''J Biol Chem.''  '''Jul 14;270(28):''' 16701-13. [http://www.ncbi.nlm.nih.gov/sites/entrez/7622480 PubMed]
# Horn C, Bremer E, Schmitt L. (2005) Functional overexpression and in vitro re-association of OpuA, an osmotically regulated ABC-transport complex from Bacillus subtilis. ''FEBS Lett.'' '''Oct 24;579(25):''' 5765-8. [http://www.ncbi.nlm.nih.gov/sites/entrez/16225868 PubMed]
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Latest revision as of 09:56, 12 July 2014