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| * '''Description:''' trigger enzyme: fructose-specific phosphotransferase system, EIIB component <br/><br/>
| | {{#bank: LevE}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''levE''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''sacL ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || trigger enzyme: fructose-specific phosphotransferase system, EIIB component
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| fructose-specific enzyme IIB (EC 2.7.1.69)
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| |style="background:#ABCDEF;" align="center"|'''Function''' || fructose uptake and phosphorylation, control of LevR activity
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 9.444
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 486 bp, 162 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[levF]]'', ''[[levD]]''
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| |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/levE_nucleotide.txt Gene sequence (+200bp) ]'''
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| |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/levE_protein.txt Protein sequence]'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:levE_context.gif]]
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| |}
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| __TOC__
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| <br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Coordinates:'''
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10317]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''Interactions:'''
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| * '''Localization:'''
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| === Database entries ===
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| * '''Structure:'''
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| * '''Swiss prot entry:'''
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| * '''KEGG entry:'''
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[levD]]-[[levE]]-[[levF]]-[[levG]]-[[sacC]]''
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| * '''Sigma factor:''' [[SigL]]
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| * '''Regulation:''' carbon catabolite repression, induction by fructose
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| * '''Regulatory mechanism:''' catabolite repression: transcription repression by [[CcpA]], transcription activator [[LevR]] is less active in the presence of glucose; induction: transcription activation by [[LevR]]
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| # Martin-Verstraete, I., Débarbouillé, M., Klier, A., and Rapoport, G. (1990) Levanase operon of ''Bacillus subtilis '' includes a fructose-specific phosphotransferase system regulating the expression of the operon. J Mol Biol 214: 657-671. [http://www.ncbi.nlm.nih.gov/sites/entrez/2117666 PubMed]
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| # Martin-Verstraete, I., Charrier, V., Stülke, J., Galinier, A., Erni, B., Rapoport, G., & Deutscher, J. (1998) Antagonistic effects of dual PTS catalyzed phosphorylation on the ''Bacillus subtilis'' transcriptional activator LevR. Mol. Microbiol. 28: 293-303. [http://www.ncbi.nlm.nih.gov/sites/entrez/9622354 PubMed]
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| # Schauder S, Nunn RS, Lanz R, Erni B, Schirmer T. 1998. Crystal structure of the IIB subunit of a fructose permease (IIBLev) from ''Bacillus subtilis''. J. Mol. Biol. 276:591-602. [http://www.ncbi.nlm.nih.gov/sites/entrez/9551099 PubMed]
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| # Seip, S., R. Lanz, R. Gutknecht, K. Flükiger, and B. Erni. 1997. The fructose transporter of Bacillus subtilis encoded by the lev operon. Backbone assignment and secondary structure of the IIBLev subunit. Eur. J. Biochem. 243: 306-314. [http://www.ncbi.nlm.nih.gov/sites/entrez/9030753 PubMed]
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| # Charrier V, Deutscher J, Martin-Verstraete I (1997b) Protein phosphorylation chain of a Bacillus subtilis fructose-specific phosphotransferase system and its participation in regulation of the expression of the lev operon. Biochemistry 36:1163-1172. [http://www.ncbi.nlm.nih.gov/sites/entrez/9033408 PubMed]
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| # Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
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