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| * '''Description:''' membrane-associated scaffold protein, orchestration of physiological processes in lipid microdomains, involved in the control of membrane fluidity, confers (together with [[YuaF]]) resistance to cefuroxime <br/><br/>
| | {{#bank: FloT}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''floT''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yuaG, yuaH ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || membrane-associated scaffold protein
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| |style="background:#ABCDEF;" align="center"|'''Function''' || involved in the control of membrane fluidity
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31010 floT]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=FloT FloT]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 55 kDa, 5.135
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1527 bp, 509 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yuaI]]'', ''[[yuaF]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31010 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31010 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31010 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:yuaG_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |-
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yuaG_3180465_3181994_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:floT_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31010]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[biofilm formation]]}},
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| {{SubtiWiki category|[[membrane dynamics]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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| {{SubtiWiki category|[[sporulation/ other]]}},
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| {{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[SigW regulon]]}}
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|
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU31010
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| ===Phenotypes of a mutant ===
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| ** delayed onset of sporulation, reduced sporulation frequency
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| ** defect in motility {{PubMed|22753055}}
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| ** reduced [[protein secretion]] {{PubMed|23651456}}
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| ** a ''[[floT]] [[floA]]'' double mutant does not induce [[KinC]]-dependent biofilm formation upon addition of surfactin {{PubMed|20713508}}
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| ** a ''[[floT]] [[floA]]'' double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency {{PubMed|22753055}}
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| ** a ''[[floT]] [[floA]]'' double mutant has a [[sporulation]] defect, due to the lack of [[FtsH]] {{PubMed|22882210}}
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| ** a ''[[floT]]'' mutant displays a defective growth under oxygen-limiting conditions {{PubMed|25909364}}
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|
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU31010&redirect=T BSU31010]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yuaFGI.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13947]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** [[SigW]]-dependent expression of ''[[fabF]]'' and the ''[[yuaF]]-[[floT]]-[[yuaI]]'' operon result in reduced membrane fluidity {{PubMed|21542858,22178969}}
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| ** recruits [[YuaF]] to focal assemblies {{PubMed|22753055}}
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| ** controls protease activity of [[FtsH]] {{PubMed|24222488}}
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** forms homo-oligomers {{PubMed|23651456}}
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| ** oligomerization depends on the glutamate-alanine repeats {{PubMed|25909364}}
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| ** [[YuaF]]-[[FloT]] {{PubMed|22753055}}
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| ** [[FloT]]-[[FtsH]] {{PubMed|26297017,22882210}}
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| ** [[FloT]]-[[FloA]] {{PubMed|26297017,23651456}}
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| ** [[FloT]]-[[SecY]] {{PubMed|23651456}}
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| ** [[FloT]]-[[FtsX]] {{PubMed|23651456}}
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| ** [[FloT]]-[[OppA]] {{PubMed|23651456}}
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| ** [[FloT]]-[[SdhA]] {{PubMed|23651456}}
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| ** [[FloT]]-[[ResE]] {{PubMed|26297017,25909364}}
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| ** [[FloT]]-[[KinC]] to stimulate KinC dimerization and activity {{PubMed|26297017}}
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| ** [[FloT]]-[[KinD]] {{PubMed|26297017}}
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| * '''[[Localization]]:'''
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| ** membrane associated [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
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| ** co-localizes with [[FloA]] and [[KinC]] in discrete foci membrane {{PubMed|20713508}}
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| ** forms discrete focal structures in the cytoplasma membrane {{PubMed|22753055}}
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| ** 6 foci [[FloT]] of are detected in strain 3610 {{PubMed|25909364}}
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|
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU31010&redirect=T BSU31010]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O32076 O32076]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU31010]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[yuaF]]-[[floT]]-[[yuaI]]'' {{PubMed|9987136}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yuaG_3180465_3181994_-1 floT] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigW]] {{PubMed|9987136}}
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| * '''Regulation:'''
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| ** expressed upon cell wall stress ([[SigW]]) {{PubMed|9987136}}
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| ** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 1215 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 236 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** JS152 (markerless), available in [[Daniel Lopez]]'s lab
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| ** GK38 (168 ''amyE''::P''floT-yfp'' (spc)), available in [[Daniel Lopez]]'s lab
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| ** JS280 (3610 ''amyE::floT-gfp''(spc)), available in [[Daniel Lopez]]'s lab
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| ** JS153 (3610 ''lacA::floT''-mEos2 (mls)), available in [[Daniel Lopez]]'s lab
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| ** JS166 (3610 ''lacA::floT''-PAmCherry (mls)), available in [[Daniel Lopez]]'s lab
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| * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]) for FloT {{PubMed|25909364}}, available in [[Daniel Lopez]]'s lab
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| == Reviews ==
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| <pubmed> 25652542</pubmed>
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| == Original publications ==
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| <pubmed>9987136,22753055, 12107147, 18763711, 19383680 20713508 23651456 22178969 ,23249255 22882210 25635948 25909364 24222488 26297017</pubmed>
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| [[Category:Protein-coding genes]]
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