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| * '''Description:''' chromosome positioning near the pole and transport through the polar septum / antagonist of [[ParA]]-dependent inhibition of [[sporulation]] initiation, traps DNA loops, required for the recruitment of the [[condensin|SMC complex]] to the origin of replication <br/><br/>
| | {{#bank: ParB}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''parB''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''spo0J, spo0JB ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || site-specific DNA-binding protein
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || chromosome positioning before asymmetric septation,
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| specifying its orientation, and imposing directionality on
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| its subsequent transport through the septum; required for sporulation
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| (antagonist of [[Soj]]-dependent inhibition of sporulation initiation)
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40960 parB]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=ParB ParB]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 8.417
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 846 bp, 282 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yyaC]]'', ''[[parA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU40960 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU40960 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU40960 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:spo0J_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=parB_4205556_4206404_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:spo0J_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40960]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[DNA condensation/ segregation]]}},
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| {{SubtiWiki category|[[sporulation/ other]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[Spo0A regulon]]}}
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|
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU40960
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|
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU40960&redirect=T BSU40960]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/soj-spo0J.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10054]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** inhibits [[ParA]] dimerization and concomitant DNA-binding activity by stimulating [[ParA]] ATPase activity {{PubMed|21235642}}
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| ** recruits the condensin complex to the DNA origin region {{PubMed|24440393}}
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| ** the dimer binds specifcally to the centromere-like ''parS'' sequence {{PubMed|25572315}}
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| * '''Protein family:''' parB family (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[Noc]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** forms dimers {{PubMed|24829297}}
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| ** [[ParA]]-[[ParB]] {{PubMed|21235642}}
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| * '''[[Localization]]:'''
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| ** chromosome centromer {{PubMed|23475963}}
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|
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU40960&redirect=T BSU40960]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=4UMK 4UMK] (complex with ''parS'' DNA)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P26497 P26497]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU40960]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[parA]]-[[parB]]'' {{PubMed|22383849}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=parB_4205556_4206404_-1 parB] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| ** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
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| * '''Regulatory mechanism:'''
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| ** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 341 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 362 {{PubMed|24696501}}
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| ** number of protein molecules per cell: 880 (equivalent to 440 dimers) {{PubMed|24829297}}
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|
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| =Biological materials =
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| * '''Mutant:'''
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| ** 1S135 ( ''spo0J''::''spec''), {{PubMed| }}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S135&Search=1S135 BGSC]
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| ** 1S136 ( ''spo0J''::''spec''), {{PubMed| }}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S136&Search=1S136 BGSC]
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| ** 1S137 ( ''spo0J''::''spec''), {{PubMed| }}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S137&Search=1S137 BGSC]
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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|
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| =Labs working on this gene/protein=
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| * [[Heath Murray]], Centre for Bacterial Cell Biology, Newcastle, UK [http://www.ncl.ac.uk/camb/staff/profile/heath.murray homepage]
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| =Your additional remarks=
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| =References=
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| == Reviews ==
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| <pubmed> 22934648 </pubmed>
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| == Original publications ==
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| <pubmed>17462018 16677298 12562803, 10852876, 10482533, 9506522, 8071208, 17932079 19450517 21235642 26253537 9663676, 11532141, 19450516,15659156, 18854156,9159397,8866474,9778525,16925562,1900505,9364919,9701805,12950914,,14651647, 19450517, 19450516, 14563866 23475963 21911367 24440393 24829297 24696501 25071173 25572315 25951515</pubmed>
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| [[Category:Protein-coding genes]]
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