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| * '''Description:''' [[two-component systems|two-component]] sensor kinase, phosphorylates [[Spo0F]] and [[Spo0A]], part of the [[phosphorelay]], governs expression of genes involved in [[biofilm formation]] <br/><br/>
| | {{#bank: KinC}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''kinC''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ssb ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[two-component systems|two-component]] sensor kinase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || initiation of [[sporulation]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14490 kinC]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=KinC KinC]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=kinC kinC]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 48 kDa, 6.225
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1284 bp, 428 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[abh]]'', ''[[ykqA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU14490 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU14490 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU14490 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:kinC_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=kinC_1518333_1519619_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:kinC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14490]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[protein modification]]}},
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| {{SubtiWiki category|[[transcription factors and their control]]}},
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| {{SubtiWiki category|[[phosphorelay]]}},
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| {{SubtiWiki category|[[biofilm formation]]}},
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| {{SubtiWiki category|[[membrane proteins]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[Spo0A regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU14490
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| ===Phenotypes of a mutant ===
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| ** defective in [[biofilm formation]] {{PubMed|22882210}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU14490&redirect=T BSU14490]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/kinC.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10989]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** autophosphorylation, phosphorylation of [[Spo0F]] as part of the [[phosphorelay]], but also direct phosphorylation of [[Spo0A]] {{PubMed|19114652}}
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| ** mainly active in the younger, outer regions of a colony (with [[KinD]]) {{PubMed|21097618}}
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| ** phosphorylates [[Spo0A]] in response to the presence of surfactin {{PubMed|22882210}}, this has been refuted {{PubMed|25701730}}
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| ** required for initiation of [[sliding]] together with [[KinB]] {{PubMed|26152584}}
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| ** two transmembrane segments
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| ** [[PAS domain]]
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| ** C-terminal histidine phosphotransferase domain
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| * '''Modification:''' autophosphorylation on a His residue
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| ** activity is triggered by potassium leakage {{PubMed|19114652}}, this has been refuted {{PubMed|25701730}}
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| ** activity is triggered by polyisoprenoid lipids formed by [[YisP]] {{PubMed|20713508}}
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[KinC]]-[[Spo0A]] {{PubMed|19114652}}
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| ** [[KinC]]-[[Spo0F]]
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| * '''[[Localization]]:'''
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| ** cell membrane (Heterogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
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| ** co-localizes with [[FloT]] in discrete foci in the membrane {{PubMed|20713508}}
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| ** the localization of [[KinC]] in membrane microdomains depends on [[FloA]] and [[FloT]] {{PubMed|22882210}}, this has been refuted {{PubMed|25701730}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU14490&redirect=T BSU14490]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P39764 P39764]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU14490]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' kinC (according to [http://dbtbs.hgc.jp/COG/prom/kinC.html DBTBS])
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=kinC_1518333_1519619_1 kinC] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] {{PubMed|8002614}}
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| * '''Regulation:'''
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| ** expressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8002615 PubMed]
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| * '''Regulatory mechanism:'''
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| ** [[Spo0A]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/8002615 PubMed]
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| ** 1A632 ( ''kinC''::''erm''), {{PubMed|3015878}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A632&Search=1A632 BGSC]
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| ** BAL393 (''kinC''::''spc''){{PubMed|26152584}}
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| == Reviews ==
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| <pubmed>25934647 25652542</pubmed>
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| == Original publications ==
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| <pubmed>26152584,19114652,10094672,11069677,16166384, 20713508,8002615, 16479537 8002614 20946851 20971918 21097618 22882210 23927765 25701730 26152584</pubmed>
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| [[Category:Protein-coding genes]]
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