|
|
| Line 1: |
Line 1: |
| * '''Description:''' [[two-component systems|two-component]] response regulator, modulation of flagellar switch bias <br/><br/>
| | {{#bank: CheY}} |
|
| |
|
| {| align="right" border="1" cellpadding="2"
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''cheY''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''cheB ''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || [[two-component systems|two-component]] response regulator
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || modulation of flagellar switch bias
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16330 cheY]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=CheY CheY]
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 13 kDa, 4.746
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 360 bp, 120 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fliY]]'', ''[[fliZ]]''
| |
| |-
| |
| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU16330 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU16330 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU16330 DNA_with_flanks]
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:cheY_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cheY_1703834_1704196_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:cheY_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16330]]
| |
| |-
| |
| |}
| |
|
| |
| __TOC__
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/>
| |
|
| |
| = [[Categories]] containing this gene/protein =
| |
| {{SubtiWiki category|[[transcription factors and their control]]}},
| |
| {{SubtiWiki category|[[motility and chemotaxis]]}},
| |
| {{SubtiWiki category|[[phosphoproteins]]}}
| |
|
| |
| = This gene is a member of the following [[regulons]] =
| |
| {{SubtiWiki regulon|[[CodY regulon]]}},
| |
| {{SubtiWiki regulon|[[DegU regulon]]}},
| |
| {{SubtiWiki regulon|[[SigD regulon]]}},
| |
| {{SubtiWiki regulon|[[Spo0A regulon]]}}
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU16330
| |
|
| |
| ===Phenotypes of a mutant ===
| |
| * not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation {{PubMed|26122431}}
| |
|
| |
| === Database entries ===
| |
| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16330&redirect=T BSU16330]
| |
|
| |
| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fla-che.html]
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10258]
| |
|
| |
| === Additional information===
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:'''
| |
|
| |
| * '''Protein family:'''
| |
|
| |
| * '''Paralogous protein(s):''' [[YneI]]
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''[[Domains]]:'''
| |
|
| |
| * '''Modification:'''
| |
| ** phosphorylated by [[CheA]] on an Asp residue
| |
| ** dephosphorylated by [[CheC]] {{PubMed|23226535}}
| |
|
| |
| * '''[[Cofactors]]:'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''[[SubtInteract|Interactions]]:'''
| |
| ** [[CheY]]-P-[[CheC]] {{PubMed|23226535}}
| |
| ** [[CheA]]-[[CheY]]
| |
|
| |
| * '''[[Localization]]:'''
| |
| ** cytoplasm (according to Swiss-Prot)
| |
|
| |
| === Database entries ===
| |
| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16330&redirect=T BSU16330]
| |
|
| |
| * '''Structure:''' [http://pdb.org/pdb/explore/explore.do?structureId=1tmy 1TMY] ([[CheY]] from ''Thermotoga maritima'')
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/P24072 P24072]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16330]
| |
|
| |
| * '''E.C. number:'''
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
| * '''Operon:''' part of the [[fla-che operon]]
| |
| ** ''[[flgB]]-[[flgC]]-[[fliE]]-[[fliF]]-[[fliG]]-[[fliH]]-[[fliI]]-[[fliJ]]-[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|9657996,8157612,15175317}}
| |
| ** ''[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|20233303}}
| |
|
| |
| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cheY_1703834_1704196_1 cheY] {{PubMed|22383849}}
| |
|
| |
| * '''[[Sigma factor]]:'''
| |
| ** ''[[flgB]]'': [[SigA]] {{PubMed|9657996}}, [[SigD]] {{PubMed|9657996}}
| |
| ** ''[[ylxF]]'': [[SigD]] {{PubMed|20233303}}
| |
|
| |
| * '''Regulation:''' see [[fla-che operon]]
| |
|
| |
| * '''Regulatory mechanism:''' see [[fla-che operon]]
| |
|
| |
| * '''Additional information:'''
| |
| ** in minimal medium, CheY is present with 7,100 +/- 1,000 molecules per cell {{PubMed|21515776}}
| |
| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 1164 {{PubMed|24696501}}
| |
| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 2117 {{PubMed|24696501}}
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:'''
| |
| ** 1A798 ( ''cheY''::''cat''), {{PubMed|1905718}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A798&Search=1A798 BGSC]
| |
| ** DS6870 (marker-less in NCIB3610) {{PubMed|25313396}}
| |
| ** TB191 ''amyE''::Phy-''sfgfp'' (marker-less in NCIB3610 with constitutive expressed ''sfgfp'') {{PubMed|26122431}}
| |
| ** TB207 ''amyE''::Phy-''mKATE2'' (marker-less in NCIB3610 with constitutive expressed ''mKATE2'') {{PubMed|26122431}}
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
| ==Reviews==
| |
| <pubmed>25313396, 26122431, 19494578,,10094672 20122866 16224120 </pubmed>
| |
|
| |
| ==Original Publications==
| |
| <pubmed>,17908686,10196193,17850253,12920116,8369293,9194713,14651647, 9657996,8157612,15175317, 14749334,20080618,17675386 21515776 24386445</pubmed>
| |
|
| |
| [[Category:Protein-coding genes]]
| |
