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| * '''Description:''' Holliday junction resolvase, DNA repair, homologous recombination and chromosome segregation; recognizes, distorts, and cleaves four-stranded recombination intermediates and modulates [[RecA]] activities, equivalent to ''E. coli'' RecU, required for efficient survival and replication restart after replication-transcription conflicts<br/><br/>
| | {{#bank: RecU}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''recU''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''recG, prfA, yppB, jopB ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || Holliday junction resolvase
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || [[DNA repair/ recombination]] and chromosome segregation
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22310 recU]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RecU RecU]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 23 kDa, 9.511
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 618 bp, 206 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yppC]]'', ''[[ponA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU22310 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU22310 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU22310 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:recU_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=recU_2340802_2341422_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:recU_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22310]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[DNA repair/ recombination]]}},
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| {{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[SigM regulon]]}}
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|
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| =The gene=
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|
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| === Basic information ===
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| * '''Locus tag:''' BSU22310
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| ===Phenotypes of a mutant ===
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| * the inactivations of ''[[recU]]'' and ''[[ruvA]]-[[ruvB]]'' are synthetically lethal {{PubMed|24770420}}
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|
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU22310&redirect=T BSU22310]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/recU-ponA.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10953]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** binds Holliday junctions with high affinity {{PubMed|24770420}}
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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|
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| ** [[RecU]]-mediated Holliday junction resolution is stimulated by [[RuvB]]-ATP {{PubMed|24770420}}
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| * '''[[SubtInteract|Interactions]]:'''
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| ** form dimers {{PubMed|21600217}}
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| ** [[RecA]]-[[RecU]] {{PubMed|16024744}}
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| ** [[RecU]]-[[RuvB]] {{PubMed|24770420,21600217}}
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| ** formation of a ternary complex [[RecU]]-[[RuvB]]-Holliday junction DNA {{PubMed|24770420}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU22310&redirect=T BSU22310]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1ZP7 1ZP7]
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P39792 P39792]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU22310]
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| * '''E.C. number:'''
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|
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[recU]]-[[ponA]]'' {{PubMed|7814321}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=recU_2340802_2341422_1 recU] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigM]] {{PubMed|18179421}}
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| ** GP891 (''recU''::''cat''), available in [[Jörg Stülke]]'s lab
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| ** 1A895 (no resistance), {{PubMed|16020779}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A895&Search=1A895 BGSC]
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| == Reviews ==
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| <pubmed> 22933559 9442895</pubmed>
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| == Original publications ==
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| <pubmed>9642195,16024744,15317759,11810266,19730681 , 19422832 7814321 18179421 16154091 21600217 18684995 16020779 24770420 24792171 25939832</pubmed>
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|
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| ==RecU in other organisms==
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| <pubmed>23356868 </pubmed>
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| [[Category:Protein-coding genes]]
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