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| * '''Description:''' curvature sensitive membrane binding protein that recruits other proteins to the poles and the division septum, cell-division initiation protein (septum placement), part of the Min system (with [[MinC]], [[MinD]], [[MinJ]]), [[Noc]] and the Min system ensure the efficient utilization of the division site at midcell in by ensuring [[FtsZ|Z ring]] placement <br/><br/>
| | {{#bank: DivIVA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''divIVA''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmJ ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || cell-division initiation protein
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || septum placement
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15420 divIVA]
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=DivIVA DivIVA]
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| |-
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 4.846
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 492 bp, 164 aa
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmH]]'', ''[[ileS]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15420 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15420 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15420 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:divIVA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |-
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=divIVA_1612521_1613015_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:divIVA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15420]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[cell division]]}},
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| {{SubtiWiki category|[[membrane proteins]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[Spo0A regulon]]}}
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|
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| =The gene=
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|
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| === Basic information ===
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| * '''Locus tag:''' BSU15420
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| ===Phenotypes of a mutant ===
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| * Deletion of ''divIVA'' leads to filamentation and polar divisions that in turn cause a minicell phenotype. [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed]
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| * A ''divIVA'' mutant has a severe [[sporulation]] defect. [http://www.ncbi.nlm.nih.gov/sites/entrez/11445541 PubMed]
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|
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15420&redirect=T BSU15420]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/divIVA.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11835]
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| === Additional information===
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| Filamentation is suppressed by mutations in minCD [http://www.ncbi.nlm.nih.gov/sites/entrez/9045828 PubMed].
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|
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| =The protein=
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|
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** curvature sensitive membrane binding protein that recruits other proteins to the poles and the division septum
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| ** DivIVA is required for polar localisation of [[MinC]]-[[MinD]] via [[MinJ]]. [http://www.ncbi.nlm.nih.gov/sites/entrez/19019154 PubMed]
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| ** It also recruits [[RacA]] to the distal pole of the prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/12493822 PubMed].
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| ** DivIVA may anchor [[SpoIIE]] briefly to the assembling polar septum before [[SpoIIE]] is subsequently released into the forespore membrane and recaptured at the polar septum {{PubMed|25101664}}
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| ** required for the compartment-specific activation of [[SigF]] {{PubMed|25101664}}
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| * '''Protein family:''' gpsB family (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[GpsB]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| ** the first 60 amino acids constitute a conserved lipid binding domain. [http://www.ncbi.nlm.nih.gov/sites/entrez/19478798 PubMed]
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| ** the C-terminal domain is less conserved
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| ** multimerisation involves two coiled-coil motifs, one in the lipid binding domain, and the other one being present in the helical C-terminal domain {{PubMed|18388125}} {{PubMed|23264578}}
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| * '''Modification:'''
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| ** phosphorylated on Arg-102 {{PubMed|22517742}}
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| ** The ''Mycobacterium'' DivIVA homologue Wag31 is phosphorylated at T73 [http://www.ncbi.nlm.nih.gov/sites/entrez/15985609 PubMed]
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| ** DivIVA from ''Streptococcus pneumoniae'' is phosphorylated at Threonine 201 by the Ser/Thr protein kinase Sktp1. [http://www.ncbi.nlm.nih.gov/sites/entrez/20453092 PubMed][http://www.ncbi.nlm.nih.gov/sites/entrez/22211696 PubMed]
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| * '''[[Cofactors]]:''' not known
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| * '''Effectors of protein activity:''' not known
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| * '''[[SubtInteract|Interactions]]:'''
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| ** ([[MinC]]-[[MinD]])-[[MinJ]]-[[DivIVA]] {{PubMed|20352045}}, [[MinJ]] binds to the N-terminal lipid-binding domain of [[DivIVA]] {{PubMed|23264578}}
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| ** [[DivIVA]]-[[RacA]], [[RacA]] binds to the C-terminal domain of [[DivIVA]] {{PubMed|23264578}}
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| ** [[DivIVA]]-[[DivIVA]] {{PubMed|23264578}}
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| ** [[Maf]]-[[DivIVA]] {{PubMed|21564336}}
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| ** [[DivIVA]]-[[ComN]] {{PubMed|22582279}}
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| ** [[DivIVA]]-[[SecA]] {{PubMed|24592260}}
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| ** [[DivIVA]]-[[SpoIIE]] {{PubMed|25101664}}
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| * '''[[Localization]]:'''
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| ** DivIVA forms a ring underneath the invaginating membrane at the site of cell division and is enriched at both cell poles [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed].
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| ** forms rings at the division septum and patches at the cell poles {{PubMed|22108385}}
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| ** membrane targeting requires [[SecA]] {{PubMed|24592260}}
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| ** assembles into a ring-like structure at the polar septum during [[sporulation]] {{PubMed|25101664}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15420&redirect=T BSU15420]
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| * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=2WUJ 2WUJ] (N-terminal domain) {{PubMed|20502438}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P71021 P71021]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15420]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''divIVA'' [http://www.ncbi.nlm.nih.gov/sites/entrez/16420366 PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=divIVA_1612521_1613015_1 divIVA] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| ** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
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| ** constitutively expressed {{PubMed|23701187}}
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| * '''Regulatory mechanism:'''
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| ** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 1595 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 3553 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** 4041 (''divIVA''::''tet''), available in [[Leendert Hamoen]]'s, [[Jörg Stülke]]'s, and [[Sven Halbedel]] 's lab
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| ** GP1482 (chromosomal ''divIVA''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [[SPINE]], available in [[Jörg Stülke]]'s lab'''
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| * '''Expression vector:'''
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| ** DivIVA-Strep available [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html here]
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| ** pGP1497 (N-terminal Strep-tag fused to C-terminus of ''divIVA'', TEV-site purification from ''E. coli'', in [[pGP172]]), available in [[Jörg Stülke]]'s lab
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| * '''lacZ fusion:'''
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| * '''GFP fusion:''' divIVA-gfp fusions available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab
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| * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Sven Halbedel]]'s and [[Jörg Stülke]]'s labs
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| * '''Antibody:''' A polyclonal anti-DivIVA antiserum generated in rabbit is described here {{PubMed|11445541}}.
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| =Labs working on this gene/protein=
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| [[Leendert Hamoen]], Centre for Bacterial Cell Biology, Newcastle upon Tyne, United Kingdom [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen]
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| [[Imrich Barak]], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]
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| [[Sven Halbedel]], Robert Koch Institute [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html homepage]
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 19654604, 19884039 22722244 23182676 </pubmed>
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| ==Original Publications==
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| <pubmed>22582279, 19654604, 19666580,9219999,19019154,15554965, 12368265,11445541,10835369,12511520,14651647, 19478798 ,19429628, 11445541, 9219999, 9045828 20352045 20502438 11886553 21564336 22108385 22457634 22517742 22661688 23264578 23701187 23927765 24391905 24592260 24600441 25101664 </pubmed>
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| [[Category:Protein-coding genes]]
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