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| * '''Description:''' [[two-component systems|two-component]] response regulator, modulation of flagellar switch bias <br/><br/>
| | {{#bank: CheY}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''cheY''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''cheB ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[two-component systems|two-component]] response regulator
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || modulation of flagellar switch bias
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16330 cheY]
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=CheY CheY]
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| |-
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 13 kDa, 4.746
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 360 bp, 120 aa
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fliY]]'', ''[[fliZ]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU16330 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU16330 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU16330 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:cheY_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |-
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cheY_1703834_1704196_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:cheY_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16330]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[transcription factors and their control]]}},
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| {{SubtiWiki category|[[motility and chemotaxis]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CodY regulon]]}},
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| {{SubtiWiki regulon|[[DegU regulon]]}},
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| {{SubtiWiki regulon|[[SigD regulon]]}},
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| {{SubtiWiki regulon|[[Spo0A regulon]]}}
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|
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU16330
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16330&redirect=T BSU16330]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fla-che.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10258]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:'''
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| * '''Paralogous protein(s):''' [[YneI]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| ** phosphorylated by [[CheA]] on an Asp residue
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| ** dephosphorylated by [[CheC]] {{PubMed|23226535}}
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[CheY]]-P-[[CheC]] {{PubMed|23226535}}
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| ** [[CheA]]-[[CheY]]
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| * '''[[Localization]]:'''
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| ** cytoplasm (according to Swiss-Prot)
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16330&redirect=T BSU16330]
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| * '''Structure:''' [http://pdb.org/pdb/explore/explore.do?structureId=1tmy 1TMY] ([[CheY]] from ''Thermotoga maritima'')
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P24072 P24072]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16330]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' part of the [[fla-che operon]]
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| ** ''[[flgB]]-[[flgC]]-[[fliE]]-[[fliF]]-[[fliG]]-[[fliH]]-[[fliI]]-[[fliJ]]-[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|9657996,8157612,15175317}}
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| ** ''[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|20233303}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cheY_1703834_1704196_1 cheY] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| ** ''[[flgB]]'': [[SigA]] {{PubMed|9657996}}, [[SigD]] {{PubMed|9657996}}
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| ** ''[[ylxF]]'': [[SigD]] {{PubMed|20233303}}
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| * '''Regulation:''' see [[fla-che operon]]
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| * '''Regulatory mechanism:''' see [[fla-che operon]]
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| * '''Additional information:'''
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| ** in minimal medium, CheY is present with 7,100 +/- 1,000 molecules per cell {{PubMed|21515776}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 1164 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 2117 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** 1A798 ( ''cheY''::''cat''), {{PubMed|1905718}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A798&Search=1A798 BGSC]
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>19494578,,10094672 20122866 16224120 </pubmed>
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| ==Original Publications==
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| <pubmed>,17908686,10196193,17850253,12920116,8369293,9194713,14651647, 9657996,8157612,15175317, 14749334,20080618,17675386 21515776 24386445</pubmed>
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| [[Category:Protein-coding genes]]
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