|
|
| (2 intermediate revisions by 2 users not shown) |
| Line 1: |
Line 1: |
| * '''Description:''' flagellar basal-body M-ring protein, membrane anchor of the basal body <br/><br/>
| | {{#bank: FliF}} |
|
| |
|
| {| align="right" border="1" cellpadding="2"
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''fliF''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || flagellar basal-body M-ring protein
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || movement and chemotaxis
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16210 fliF]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=FliF FliF]
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 59 kDa, 4.37
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1608 bp, 536 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fliE]]'', ''[[fliG]]''
| |
| |-
| |
| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU16210 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU16210 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU16210 DNA_with_flanks]
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:fliF_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fliF_1692496_1694106_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:fliF_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16210]]
| |
| |-
| |
| |}
| |
|
| |
| __TOC__
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/>
| |
|
| |
| = [[Categories]] containing this gene/protein =
| |
| {{SubtiWiki category|[[motility and chemotaxis]]}},
| |
| {{SubtiWiki category|[[membrane proteins]]}}
| |
|
| |
| = This gene is a member of the following [[regulons]] =
| |
| {{SubtiWiki regulon|[[CodY regulon]]}},
| |
| {{SubtiWiki regulon|[[DegU regulon]]}},
| |
| {{SubtiWiki regulon|[[SigD regulon]]}},
| |
| {{SubtiWiki regulon|[[Spo0A regulon]]}}
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU16210
| |
|
| |
| ===Phenotypes of a mutant ===
| |
| * mucoid phenotype due to the overproduction of poly-gamma-glutamate {{PubMed|24296669}}
| |
| * no secretion of [[FlgM]], permanent inhibition of [[SigD]] {{PubMed|25313396}}
| |
|
| |
| === Database entries ===
| |
| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16210&redirect=T BSU16210]
| |
|
| |
| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fla-che.html]
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10240]
| |
|
| |
| === Additional information===
| |
|
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:'''
| |
| ** required for correct localization of [[FliM]] {{PubMed|23190039}}
| |
|
| |
| * '''Protein family:''' fliF family (according to Swiss-Prot)
| |
|
| |
| * '''Paralogous protein(s):'''
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''[[Domains]]:'''
| |
|
| |
| * '''Modification:'''
| |
|
| |
| * '''[[Cofactors]]:'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''[[SubtInteract|Interactions]]:'''
| |
| ** [[FliF]]-[[FliG]] {{PubMed|25313396}}
| |
|
| |
| * '''[[Localization]]:'''
| |
| ** cell membrane [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
| |
|
| |
| === Database entries ===
| |
| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16210&redirect=T BSU16210]
| |
|
| |
| * '''Structure:'''
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/P23447 P23447]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16210]
| |
|
| |
| * '''E.C. number:'''
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
|
| |
| * '''Operon:''' part of the [[fla-che operon]] (see there for regulation)
| |
| ** ''[[flgB]]-[[flgC]]-[[fliE]]-[[fliF]]-[[fliG]]-[[fliH]]-[[fliI]]-[[fliJ]]-[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|9657996,8157612,15175317}}
| |
|
| |
| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fliF_1692496_1694106_1 fliF] {{PubMed|22383849}}
| |
|
| |
| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|9657996}}, [[SigD]] {{PubMed|9657996}}
| |
|
| |
| * '''Regulation:''' see [[fla-che operon]]
| |
|
| |
| * '''Regulatory mechanism:''' see [[fla-che operon]]
| |
|
| |
| * '''Additional information:'''
| |
| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 68 {{PubMed|24696501}}
| |
| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 595 {{PubMed|24696501}}
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:'''
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
|
| |
| <pubmed>1905667,17850253,14651647,18763711, 9657996,8157612,15175317 23190039 24296669 25313396 24386445</pubmed>
| |
|
| |
| [[Category:Protein-coding genes]]
| |
