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| * '''Description:''' transcriptional repressor of the peroxide regulon, sensor of the intracellular Fe/Mn ratio <br/><br/>
| | {{#bank: PerR}} |
| {| align="right" border="1" cellpadding="2" | |
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''perR''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ygaG ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional repressor ([[Fur family]])
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of the response to peroxide
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU08730 perR]
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=perR perR]'''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 16 kDa, 5.888
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 435 bp, 145 aa
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ygaF]]'', ''[[ygzB]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU08730 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU08730 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU08730 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:perR_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |-
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=perR_944487_944924_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:perR_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU08730]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[transcription factors and their control]]}},
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| {{SubtiWiki category|[[resistance against oxidative and electrophile stress]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[PerR regulon]]}}
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| =The [[PerR regulon]]=
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| =The gene=
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|
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| === Basic information ===
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| * '''Locus tag:''' BSU08730
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| ===Phenotypes of a mutant ===
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| * resistant to hydrogen peroxide, accumulates a porphyrin-like compound, and grows very slowly (due to heme sequestration by [[KatA]]) {{PubMed|22194458}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU08730&redirect=T BSU08730]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/perR.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12227]
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|
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' [[Fur family]]
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| * '''Paralogous protein(s):''' [[Zur]], [[Fur]]
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|
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| ** selective metal catalyzed oxidation of two histidine residues of the regulatory site results in induction (loss of DNA-binding activity) [http://www.ncbi.nlm.nih.gov/sites/entrez/19508285 PubMed]
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| * '''Cofactor(s):''' contains an Fe(2+) at the regulatory site and Zn(2+) {{PubMed|22797754}}
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| * '''Effectors of protein activity:'''
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| ** responds to the presence of hydrogen peroxide
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|
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
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|
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU08730&redirect=T BSU08730]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2RGV 2RGV] [http://www.rcsb.org/pdb/explore.do?structureId=3F8N 3F8N]
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P71086 P71086]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU08730]
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| * '''E.C. number:'''
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|
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''perR'' {{PubMed|12029044}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=perR_944487_944924_1 perR] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] {{PubMed|12029044}}
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| * '''Regulation:'''
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| ** negative autoregulation {{PubMed|12029044}}
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| ** induced in the presence of hydrogen peroxide ([[PerR]]) {{PubMed|12029044}}
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| * '''Regulatory mechanism:'''
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| ** [[PerR]]: transcription repression {{PubMed|12029044}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:''' HB0509 (spc), available in [[John Helmann]]'s and [[Jörg Stülke]]'s labs, also GP868 (''fur::mls'', ''perR::spc'').
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| ** 1A903 ( ''perR''::''kan''), {{PubMed|12029044}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A903&Search=1A903 BGSC]
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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|
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| =Labs working on this gene/protein=
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| [[John Helmann]], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 19575568 19508286 ,20094649 , 20977351 22797754 25160631 </pubmed>
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| ==Original Publications==
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| <pubmed> 8932315,18487332,14563870, 16766519,17158660,12486061,11532148,12180919,16166527, 15231799, 12029044,10913706, 21398634, 9701813, 16541078, 12029044, ,11532148, 12029044, 19508285 , 22194458 23645680 23057863 23934352 25486128 </pubmed>
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| [[Category:Protein-coding genes]]
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