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| * '''Description:''' [[two-component systems|two-component]] sensor kinase, phosphorylates [[Spo0F]], part of the [[phosphorelay]] <br/><br/>
| | {{#bank: KinA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''kinA''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''spoIIF, spoIIJ, gsiC, scoB, scoD ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[two-component systems|two-component]] sensor kinase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || initiation of sporulation
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13990 kinA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=KinA KinA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=kinA kinA]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 68 kDa, 5.491
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1818 bp, 606 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pbpH]]'', ''[[patA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU13990 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU13990 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU13990 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:kinA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=kinA_1470026_1471846_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:kinA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13990]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[protein modification]]}},
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| {{SubtiWiki category|[[transcription factors and their control]]}},
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| {{SubtiWiki category|[[phosphorelay]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[SigH regulon]]}},
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| {{SubtiWiki regulon|[[Spo0A regulon]]}},
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| {{SubtiWiki regulon|[[stringent response]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU13990
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13990&redirect=T BSU13990]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/kinA.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10204]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** autophosphorylation, phosphorylation of [[Spo0F]]
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| ** mainly active in the older, inner regions of a colony (with [[KinB]]) {{PubMed|21097618}}
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| ** three tandem [[PAS domain]]s in the N-terminal region of KinA, the [[second PAS]] domain is the major N-terminal determinant of KinA dimerization {{PubMed|23504013}}
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| ** the first [[PAS domain]] is required for NAD(+) binding {{PubMed|23599347}}
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| ** C-terminal histidine phosphotransferase domain
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| * '''Modification:''' autophosphorylation on a His residue
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| ** interaction with [[KipI]] or [[Sda]] inhibits autophosphorylation of [[KinA]] {{PubMed|9334321,15023339}}
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| ** autophosphorylation is inhibited by [[SivA]] and [[BslA]] {{PubMed|23335417}}
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| ** activated by interaction with NAD(+) that indicated poor respiratory activity of the cell {{PubMed|23599347}}
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| * '''[[SubtInteract|Interactions]]:'''
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| ** forms tetramers {{PubMed|21926229}}
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| ** [[KinA]]-[[KipI]] {{PubMed|9334321}}
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| ** [[KinA]]-[[Sda]] {{PubMed|15023339}}
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| ** [[Spo0F]]-[[KinA]]
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| * '''[[Localization]]:'''
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| ** cytoplasm
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13990&redirect=T BSU13990]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2VLG 2VLG] (PAS domain)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P16497 P16497]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU13990]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[kinA]]'' {{PubMed|1569009}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=kinA_1470026_1471846_1 kinA] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigH]] {{PubMed|1569009}}
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| * '''Regulation:'''
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| ** expressed under conditions that trigger sporulation ([[Spo0A]]) {{PubMed|9644251}}
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| ** induced upon addition of decoyinine (positive [[stringent response]]) {{PubMed|23378509}}
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| * '''Regulatory mechanism:'''
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| ** [[Spo0A]]: transcription activation {{PubMed|9644251}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>22303284 </pubmed>
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| ==Original Publications==
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| <pubmed>23169620 20511506 19561131, 20413551 1846779,11292798,16166384, 11734624,9299348,12067336,18174125, 10094672, 11069677,15023339,2509430, 17350039 ,9334321,9477965,9644251, 1569009 20081035 21097618 21926229 22303282 22670053 23335417 23378509 23504013 23599347 </pubmed>
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| [[Category:Protein-coding genes]]
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