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| * '''Description:''' dTDP -4-dehydrorhamnose-3,5-epimerase, spore coat polysaccharide synthesis <br/><br/>
| | {{#bank: SpsL}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''spsL''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipa-73d ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || dTDP -4-dehydrorhamnose-3,5-epimerase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || rhamnose biosynthesis, spore coat polysaccharide synthesis
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37810 spsL]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 4.878
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 453 bp, 151 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sivA]]'', ''[[spsK]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37810 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37810 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37810 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:spsL_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spsL_3882979_3883434_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:spsL_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37810]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[miscellaneous metabolic pathways]]}},
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| {{SubtiWiki category|[[sporulation proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[GerE regulon]]}},
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| {{SubtiWiki regulon|[[SigK regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU37810
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU37810&redirect=T BSU37810]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sps.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG14195]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU37810&redirect=T BSU37810]
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| * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=3RYK 3RYK] (dTDP-4-dehydrorhamnose 3,5-epimerase (''rfbC'') from ''Bacillus anthracis'' str. Ames with TDP and PPi bound, 32% identity, 56% similarity)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/Q7WY56 Q7WY56]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU37810]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/5.1.3.13 5.1.3.13]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[spsA]]-[[spsB]]-[[spsC]]-[[spsD]]-[[spsF]]-[[spsG]]-[[spsI]]-[[spsJ]]-[[spsK]]-[[spsL]]'' {{PubMed|9353933}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spsL_3882979_3883434_-1 spsL] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigK]] {{PubMed|15383836}}
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| * '''Regulation:'''
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| ** expressed late during sporulation in the mother cell ([[SigK]], [[GerE]]) {{PubMed|25239894,15383836}}
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| * '''Regulatory mechanism:'''
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| ** [[GerE]]: transcription activation {{PubMed|25239894,15383836}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>9353933 15383836 22960854 25239894</pubmed>
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| [[Category:Protein-coding genes]]
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