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| * '''Description:''' UDP-N-acetyl-D-mannosamine transferase <br/><br/>
| | {{#bank: TagA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''tagA''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed], no [http://www.ncbi.nlm.nih.gov/pubmed/19376878 PubMed]
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| |style="background:#ABCDEF;" align="center"| '''Product''' || UDP-N-acetyl-D-mannosamine transferase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of teichoic acid
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35750 tagA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=TagA TagA]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 10.133
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 768 bp, 256 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tagD]]'', ''[[tagB]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU35750 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU35750 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU35750 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:tagA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tagA_3681370_3682140_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:tagA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35750]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[cell wall synthesis]]}},
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| {{SubtiWiki category|[[biosynthesis of cell wall components]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[PhoP regulon]]}},
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| {{SubtiWiki regulon|[[WalR regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU35750
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| ===Phenotypes of a mutant ===
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| * essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed], reduced growth rate, loss of rod-like morphology [http://www.ncbi.nlm.nih.gov/pubmed/19376878 PubMed]
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| * inactivation of ''[[tagA]]'' strongly restores beta-lactam resistance in a ''[[sigM]]'' mutant {{PubMed|22211522}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35750&redirect=T BSU35750]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/tagAB.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10450]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' UDP-N-acetyl-D-mannosamine + N-acetyl-D-glucosaminyldiphosphoundecaprenol = UDP + N-acetyl-beta-D-mannosaminyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol (according to Swiss-Prot)
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| * '''Protein family:''' glycosyltransferase 26 family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| ** secreted (according to Swiss-Prot)
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35750&redirect=T BSU35750]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P27620 P27620]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU35750]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.4.1.187 2.4.1.187]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[tagA]]-[[tagB]]'' {{PubMed|7952180}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tagA_3681370_3682140_1 tagA] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] {{PubMed|7581998}}
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| * '''Regulation:'''
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| ** repressed when phosphate is available ([[PhoP]]) {{PubMed|9457886}}
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| * '''Regulatory mechanism:'''
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| ** [[PhoP]]: transcription repression {{PubMed|9457886}}
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| ** [[WalR]]: transcription activation {{PubMed|12950927}}
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 67 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 24024634 </pubmed>
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| ==Original publications==
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| <pubmed>18156271,9457886,9683503,,7952180 7581998 , 19376878 12950927 22211522</pubmed>
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| [[Category:Protein-coding genes]]
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