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| * '''Description:''' A/G-specific adenine glycosylase, error prevention oxidized guanine system, releases adenines from 8-oxo-G:A mismatches <br/><br/>
| | {{#bank: MutY}} |
| {| align="right" border="1" cellpadding="2" | |
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''mutY''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' yfhQ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || A/G-specific adenine glycosylase
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || DNA repair
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU08630 mutY]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=mutY mutY]'''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 41 kDa, 6.044
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1107 bp, 369 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yfhP]]'', ''[[yfhS]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU08630 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU08630 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU08630 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:yfhQ_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yfhQ_935656_936765_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:mutY_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU08630]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[DNA repair/ recombination]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[YfhP regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU08630
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU08630&redirect=T BSU08630]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yfhQ-fabL-sspE.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12891]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' releases adenines from 8-oxo-G:A mismatches
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU08630&redirect=T BSU08630]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1RRS 1RRS] (complex with DNA, Geobacillus stearothermophilus)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O31584 O31584]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU08630]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[mutY]]-[[fabL]]-[[sspE]]'' {{PubMed|10463184}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yfhQ_935656_936765_1 mutY] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| **[[SigA]] {{PubMed|10463184}}
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| **[[SigG]] {{PubMed|1900507}}
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| * '''Regulation:'''
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| ** expression is constitutive throughout growth {{PubMed|20971907}}
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| ** expression is fivefold increased in a ''[[mutS]]-[[mutL]]'' mutant {{PubMed|20971907}}
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| ** [[YfhP]] may act as a negative transcription regulator of the ''[[mutY]]-[[fabL]]-[[sspE]]'' operon during exponential growth {{PubMed|10463184}}
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 52 {{PubMed|24696501}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 168 {{PubMed|21395229}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 201 {{PubMed|21395229}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 215 {{PubMed|21395229}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 22933559 </pubmed>
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| == Original publications ==
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| <pubmed>10463184 19011023 20971907</pubmed>
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| [[Category:Protein-coding genes]]
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