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| * '''Description:''' soluble chemotaxis receptor, heme-containing O(2) sensor protein <br/><br/>
| | {{#bank: HemAT}} |
| {| align="right" border="1" cellpadding="2" | |
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''hemAT''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yhfV ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || haem-based aerotactic transducer
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || movement towards oxygen
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU10380 hemAT]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=HemAT HemAT]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 48 kDa, 5.441
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1296 bp, 432 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhfU]]'', ''[[yhfW]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU10380 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU10380 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU10380 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:hemAT_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=hemAT_1112620_1113918_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:hemAT_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU10380]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[motility and chemotaxis]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[SigD regulon]]}}
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|
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU10380
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU10380&redirect=T BSU10380]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/hemAT.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13066]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** required for full expression of the ''[[phoD]]-[[tatAD]]-[[tatCD]]'' operon {{PubMed|23180473}}
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):''' heme
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[HemAT]]-[[TatAC]] {{PubMed|23180473}}
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| * '''[[Localization]]:'''
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| ** forms clusters at the cell poles {{PubMed|21515776}}
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| ** homogeneous cytoplsmic distribution in chain-forming cells during the logarithmic phase {{PubMed|23180473}}
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| ** polar foci in individual cells at later growth stages {{PubMed|23180473}}
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|
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU10380&redirect=T BSU10380]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1OR4 1OR4] (in cyano-liganded form), [http://www.rcsb.org/pdb/explore.do?structureId=1OR6 1OR6]
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O07621 O07621]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU10380]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''hemAT'' {{PubMed|15033535}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=hemAT_1112620_1113918_-1 hemAT] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigD]] {{PubMed|15033535}}
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** in minimal medium, HemAT is present with 19,000 +/- 3,900 molecules per cell {{PubMed|21515776}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 1095 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 1803 {{PubMed|24696501}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 3169 {{PubMed|21395229}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 966 {{PubMed|21395229}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1236 {{PubMed|21395229}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| == Reviews ==
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| <pubmed> 23928310 </pubmed>
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| == Original publications ==
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| <pubmed>10676961,16819829,11481493,15033535 21515776 23180473 22564695</pubmed>
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| [[Category:Protein-coding genes]]
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