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| * '''Description:''' fumarase<br/><br/>
| | {{#bank: CitG}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''citG''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || fumarate hydratase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || TCA cycle
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33040 citG]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=CitG CitG]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=citG citG]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 50 kDa, 5.475
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1386 bp, 462 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yuxN]], [[yvzF]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU33040 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU33040 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU33040 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:citG_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fumC_3389024_3390412_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:citG_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33040]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[carbon core metabolism]]}},
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| [[most abundant proteins]]
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU33040
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU33040&redirect=T BSU33040]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/citG.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10384]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' (S)-malate = fumarate + H<sub>2</sub>O (according to Swiss-Prot)
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| * '''Protein family:''' Fumarase subfamily (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[AnsB]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[Mdh]]-[[CitG]] {{PubMed|20933603}}
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| ** [[CitG]]-[[CitB]] {{PubMed|20933603}}
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| * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU33040&redirect=T BSU33040]
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| * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=3R6Q 3R6Q] (aspartase from ''Bacillus'' sp. YM55-1, 43% identity, 77% similarity) {{PubMed|21661762}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P07343 P07343]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU33040]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/4.2.1.2 4.2.1.2]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[citG]]'' {{PubMed|3923430}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fumC_3389024_3390412_-1 citG] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|3130545,2509422,2509423}}, [[SigH]] {{PubMed|3130545,2509422}}
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| * '''Regulation:''' constitutive
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 2427 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 9880 {{PubMed|24696501}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 7597 {{PubMed|21395229}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 4282 {{PubMed|21395229}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 4666 {{PubMed|21395229}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** GP718 (spec), available in [[Jörg Stülke]]'s lab
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| * '''Expression vector:'''
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| ** pGP1122 (N-terminal Strep-tag, for [[SPINE]], purification from ''B. subtilis'', in [[pGP380]]) (available in [[Jörg Stülke]]'s lab)
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| * '''lacZ fusion:'''
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| ** pGP387 (in [[pAC7]]), available in [[Jörg Stülke]]'s lab
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| * '''GFP fusion:''' GP1433 (spc, based on [[pGP1870]]), available in the [[Stülke]] lab
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| * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
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| * '''Antibody:'''
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| * '''FLAG-tag construct:'''
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| ** GP1132 (spc, based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>2509422,3923430 ,3130545 2509423 21661762 20933603 15378759</pubmed>
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| [[Category:Protein-coding genes]]
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