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| * '''Description:''' elemental iron uptake system, heme peroxidase, converts ferrous iron (Fe(II) to ferric iron (FeIII)) for uptake by [[EfeO]]-[[EfeU]], peroxide detoxification under microaerobic conditions<br/><br/>
| | {{#bank: EfeB}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''efeB''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipa-29d, ywbN ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || heme peroxidase in elemental iron uptake
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || ferrous iron conversion
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU38260 efeB]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/EfeB EfeB]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=EfeB EfeB]'''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 8.64
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1248 bp, 416 aa
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywbO]]'', ''[[efeO]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU38260 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU38260 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU38260 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ywbN_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=efeN_3926682_3927932_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:ywbN_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU38260]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[acquisition of iron]]}},
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| {{SubtiWiki category|[[iron metabolism]]}},
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| {{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}},
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| {{SubtiWiki category|[[resistance against oxidative and electrophile stress]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[Fur regulon]]}},
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| {{SubtiWiki regulon|[[SigM regulon]]}},
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| {{SubtiWiki regulon|[[SigW regulon]]}},
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| {{SubtiWiki regulon|[[SigX regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU38260
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywbLMN.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10575]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** oxidizes ferrous iron to ferric iron for uptake by [[EfeO]]-[[EfeU]] {{PubMed|23764491}}
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| ** eliminates reactive oxygen species that accumulate in the presence of ferrous iron {{PubMed|23764491}}
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| ** peroxide detoxification under microaerobic conditions {{PubMed|23764491}}
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| * '''Protein family:''' DyP-type peroxidase family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| ** protoheme IX {{PubMed|23764491}}
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| * '''Effectors of protein activity:'''
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| ** activity is increased in the presence of [[EfeO]] due to the displacement of ferric iron {{PubMed|23764491}}
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[EfeO]]-[[EfeU]]-[[EfeB]] {{PubMed|23180473,23764491}}
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| * '''[[Localization]]:'''
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| ** extracellular, secreted by the [[TatAY]]-[[TatCY]] complex {{PubMed|15554971}}
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| ** [[TatAY]]-[[TatCY]]-dependent export of [[EfeB]] requires a functional [[WprA]] {{PubMed|23180473}}
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| ** forms a membrane-bound complex with [[EfeU]] and [[EfeO]] for iron uptake {{PubMed|23180473,23764491}}
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| ** attached to the membrane via [[EfeU]] {{PubMed|23764491}}
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P39597 P39597]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU38260]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| ** ''[[efeU]]-[[efeO]]-[[efeB]]'' {{PubMed|9353933}}
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| ** ''[[efeB]]-[[ywbO]]'' {{PubMed|18179421}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=efeN_3926682_3927932_-1 efeB] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| ** ''[[efeU]]'': [[SigA]] {{PubMed|23764491}}
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| ** ''[[efeB]]'': [[SigM]] {{PubMed|18179421}}, [[SigX]], [[SigW]] {{PubMed|9683469}}
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| * '''Regulation:'''
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| ** induced upon iron starvation ([[Fur]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12354229 PubMed]
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| * '''Regulatory mechanism:'''
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| ** [[Fur]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12354229 PubMed]
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 141 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Jan Maarten van Dijl]], Groningen, Netherlands
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| =Your additional remarks=
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| =References=
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| == Reviews ==
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| <pubmed> 24140208 </pubmed>
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| == Original publications ==
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| <pubmed>16672620,19180538,12354229, 19383693 9353933 9683469 15554971 21479178 18179421 22923395 23180473 23560556 23764491 23820555 24620988 18931290 </pubmed>
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| [[Category:Protein-coding genes]]
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