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| * '''Description:''' 1-pyrroline-5-carboxylate dehydrogenase <br/><br/>
| | {{#bank: PutC}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''putC''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ycgN ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || 1-pyrroline-5-carboxylate dehydrogenase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || proline utilization
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03210 putC]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=putC putC]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 56 kDa, 5.372
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1545 bp, 515 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[putB]]'', ''[[putP]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU03210 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU03210 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU03210 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ycgN_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ycgN_345479_347026_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:putC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03210]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[utilization of amino acids]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CodY regulon]]}},
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| {{SubtiWiki regulon|[[PutR regulon]]}},
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| {{SubtiWiki regulon|[[Spo0A regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU03210
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| ===Phenotypes of a mutant ===
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| * no growth with proline as single source of carbon or nitrogen {{PubMed|21840319}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU03210&redirect=T BSU03210]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ycgMN.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12012]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' (S)-1-pyrroline-5-carboxylate + NAD(P)<sup>+</sup> + 2 H<sub>2</sub>O = L-glutamate + NAD(P)H (according to Swiss-Prot)
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| * '''Protein family:''' [[RocA]] subfamily (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[RocA]], additional similar proteins: [[YfmT]], [[AldY]], [[YcbD]], [[GabD]], [[GbsA]], [[DhaS]], [[IolA]], [[AldX]], [[YwdH]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU03210&redirect=T BSU03210]
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| * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=3RJL 3RJL] (1-pyrroline-5-carboxylate dehydrogenase from ''Bacillus licheniformis'', 90% identity, 97% similarity)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P94391 P94391]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU03210]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/1.5.1.12 1.5.1.12]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[putB]]-[[putC]]-[[putP]]'' {{PubMed|21840319,22139509}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ycgN_345479_347026_1 putC] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] {{PubMed|21840319,21964733,22139509}}
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| * '''Regulation:'''
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| ** induced in the presence of proline ([[PutR]]) {{PubMed|21840319,22139509}}
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| ** expressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
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| ** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
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| ** strongly induced in response to glucose starvation in M9 medium {{PubMed|23033921}}
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| * '''Regulatory mechanism:'''
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| ** [[PutR]]: transcription activation {{PubMed|21840319,21964733,22139509}}
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| ** [[Spo0A]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
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| ** [[CodY]]: transcription repression, displacement of [[PutR]] {{PubMed|21840319}}
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| * '''Additional information:'''
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| ** overexpressed in a ''[[pnpA]]'' mutant {{PubMed|14976255}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 90 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 36819 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>12618455,14976255, 14651647 21840319 23033921,21964733,22139509</pubmed>
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| [[Category:Protein-coding genes]]
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