|
|
| (2 intermediate revisions by 2 users not shown) |
| Line 1: |
Line 1: |
| * '''Description:''' glycerol-3-phosphate cytidylyltransferase <br/><br/>
| | {{#bank: TagD}} |
|
| |
|
| {| align="right" border="1" cellpadding="2"
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''tagD''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || glycerol-3-phosphate cytidylyltransferase
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of teichoic acid
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35740 tagD]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=TagD TagD]'''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 15 kDa, 5.672
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 387 bp, 129 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tagE]]'', ''[[tagA]]''
| |
| |-
| |
| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU35740 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU35740 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU35740 DNA_with_flanks]
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:tagD_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tagD_3680581_3680970_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:tagD_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35740]]
| |
| |-
| |
| |}
| |
|
| |
| __TOC__
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
|
| |
|
| |
| <br/><br/>
| |
|
| |
| = [[Categories]] containing this gene/protein =
| |
| {{SubtiWiki category|[[cell wall synthesis]]}},
| |
| {{SubtiWiki category|[[biosynthesis of cell wall components]]}},
| |
| {{SubtiWiki category|[[essential genes]]}}
| |
|
| |
| = This gene is a member of the following [[regulons]] =
| |
| {{SubtiWiki regulon|[[PhoP regulon]]}},
| |
| {{SubtiWiki regulon|[[WalR regulon]]}}
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU35740
| |
|
| |
| ===Phenotypes of a mutant ===
| |
|
| |
| essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
| |
|
| |
| === Database entries ===
| |
| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35740&redirect=T BSU35740]
| |
|
| |
| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/tagDEF.html]
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10449]
| |
|
| |
| === Additional information===
| |
|
| |
|
| |
|
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:''' CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol (according to Swiss-Prot)
| |
|
| |
| * '''Protein family:''' cytidylyltransferase family (according to Swiss-Prot)
| |
|
| |
| * '''Paralogous protein(s):'''
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''Domains:'''
| |
|
| |
| * '''Modification:'''
| |
|
| |
| * '''Cofactor(s):'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''[[SubtInteract|Interactions]]:'''
| |
| ** [[TagD]]-[[TagD]] {{PubMed|10508782}}
| |
|
| |
| * '''[[Localization]]:'''
| |
| ** cytoplasm (according to Swiss-Prot)
| |
|
| |
| === Database entries ===
| |
| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35740&redirect=T BSU35740]
| |
|
| |
| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1N1D 1N1D] (complex with CDP-glycerol) {{PubMed|10508782}}, [http://www.rcsb.org/pdb/explore.do?structureId=1COZ 1COZ]
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/P27623 P27623]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU35740]
| |
|
| |
| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.7.39 2.7.7.39]
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
| * '''Operon:''' ''[[tagD]]-[[tagE]]-[[tagF]]'' {{PubMed|2507871}}
| |
|
| |
| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tagD_3680581_3680970_-1 tagD] {{PubMed|22383849}}
| |
|
| |
| * '''Sigma factor:''' [[SigA]] {{PubMed|7581998}}
| |
|
| |
| * '''Regulation:'''
| |
| ** repressed by phosphate ([[PhoP]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9457886 PubMed]
| |
|
| |
| * '''Regulatory mechanism:'''
| |
| ** [[PhoP]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/9457886 PubMed]
| |
| ** [[WalR]]: transcription activation {{PubMed|12950927}}
| |
|
| |
| * '''Additional information:'''
| |
| ** The mRNA has a long 5' leader region. This may indicate RNA-based regulation {{PubMed|20525796}}
| |
| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 604 {{PubMed|24696501}}
| |
| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 2215 {{PubMed|24696501}}
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:'''
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
|
| |
| <pubmed>9683503,9457886,11673441,10508782,2507871,12950927, 7581998 20525796</pubmed>
| |
|
| |
| [[Category:Protein-coding genes]]
| |
