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| * '''Description:''' [[two-component systems|two-component]] sensor kinase, chemotactic signal modulator <br/><br/>
| | {{#bank: CheA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''cheA''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''cheN ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[two-component systems|two-component]] sensor kinase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || chemotactic signal modulator
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16430 cheA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=CheA CheA]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 74 kDa, 4.452
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2013 bp, 671 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cheB]]'', ''[[cheW]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU16430 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU16430 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU16430 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:cheA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cheA_1712815_1714833_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:cheA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16430]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[protein modification]]}},
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| {{SubtiWiki category|[[transcription factors and their control]]}},
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| {{SubtiWiki category|[[motility and chemotaxis]]}},
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| {{SubtiWiki category|[[membrane proteins]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CodY regulon]]}},
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| {{SubtiWiki regulon|[[SigD regulon]]}},
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| {{SubtiWiki regulon|[[Spo0A regulon]]}}
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|
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU16430
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16430&redirect=T BSU16430]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fla-che.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10256]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' autophosphorylation, phosphorylation of [[CheY]] and [[CheB]] {{PubMed|11553614}}
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| ** autophosphorylation on a His residue
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| ** phosphorylation of [[CheA]] is stimulated by interaction with the complex of deaminated [[McpC]]-[[CheD]] {{PubMed|22931217}}
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| ** the kinase activity of [[CheA]] is activated by chemoreceptors bound to [[CheD]] {{PubMed|23226535}}
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[CheA]]-[[McpB]] {{PubMed|19864420}}
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| ** [[CheA]]-[[CheC]] {{PubMed|11722727}}
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| **[[CheA]]-[[CheY]] {{PubMed|11553614}}
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| * '''[[Localization]]:'''
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| ** membrane (according to Swiss-Prot)
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| ** localizes to the cell poles {{PubMed|21098025}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16430&redirect=T BSU16430]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P29072 P29072]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16430]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| ** ''[[flgB]]-[[flgC]]-[[fliE]]-[[fliF]]-[[fliG]]-[[fliH]]-[[fliI]]-[[fliJ]]-[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|9657996,8157612,15175317}}
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| ** ''[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|20233303}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cheA_1712815_1714833_1 cheA] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| ** ''[[flgB]]'': [[SigA]] {{PubMed|9657996}}, [[SigD]] {{PubMed|9657996}}
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| ** ''[[ylxF]]'': [[SigD]] {{PubMed|20233303}}
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| * '''Regulation:'''
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| ** repressed under conditions that trigger sporulation ([[Spo0A]]) {{PubMed|14651647}}
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| * '''Regulatory mechanism:'''
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| ** [[Spo0A]]: transcription repression {{PubMed|14651647}}
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| ** [[CodY]]: transcription repression {{PubMed|12730172}}
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| ** [[DegU]] (non-phosphorylated): transcription activation {{PubMed|17850253}}
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| * '''Additional information:'''
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| ** in minimal medium, CheA is present with 2,600 +/- 560 molecules per cell {{PubMed|21515776}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 705 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 1580 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** 1A797 ( ''cheA''::''cat''), {{PubMed|1938941}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A797&Search=1A797 BGSC]
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 20122866 </pubmed>
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| ==Original publications==
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| <pubmed>1938941,12123464,17908686,8866475,12094812,10094672,11722727,14651647, 9657996,8157612,15175317 9721285 17850253 11553614 21098025 22931217 21515776 23226535</pubmed>
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| [[Category:Protein-coding genes]]
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