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| * '''Description:''' [[ribosomal protein]] <br/><br/>
| | {{#bank: RpsF}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''rpsF''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no {{PubMed|23002217}}
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[ribosomal protein]] S6 (BS9)
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| |style="background:#ABCDEF;" align="center"|'''Function''' || [[translation]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40910 rpsF]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RpsF RpsF]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 10 kDa, 4.993
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 285 bp, 95 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ssbA]]'', ''[[yyaF]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU40910 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU40910 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU40910 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:rpsF_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rpsF_4199445_4199732_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:rpsF_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40910]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[translation]]}},
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| [[most abundant proteins]]
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[ComK regulon]]}},
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| {{SubtiWiki regulon|[[stringent response]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU40910
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU40910&redirect=T BSU40910]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yyaF-rpsF-ssb-rpsR.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10049]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' [[ribosomal protein]] S6P family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[RpsF]]-[[RpsR]], the heterodimer binds the structure in the ''[[rpsF]]'' mRNA 5' UTR {{PubMed|24310371}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU40910&redirect=T BSU40910]
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| * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=3R3T 3R3T] (from ''Bacillus anthracis'', 71% identity, 97% similarity)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P21468 P21468]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU40910]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| ** ''[[yyaF]]-[[rpsF]]-[[ssbA]]-[[rpsR]]'' {{PubMed|14762004}}
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| ** ''[[rpsF]]-[[ssbA]]-[[rpsR]]'' {{PubMed|24310371,22383849}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rpsF_4199445_4199732_-1 rpsF] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| ** ''[[rpsF]]'': [[SigA]] {{PubMed|24310371}}
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| * '''Regulation:'''
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| ** [[RelA]] dependent downregulation (Class I) during stringent response {{PubMed|11948165}}
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| ** [[ComK]]: transcription activation {{PubMed|11948146}}
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| * '''Additional information:'''
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| ** the mRNA contains a structured motif in the 5' UTR upstream of the ''[[rpsF]]'' ORF that is bound by [[RpsF]]-[[RpsR]] heterodimers and may be implicated in autogenous regulation {{PubMed|24310371}}
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| ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 3469 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 4039 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>19653700 14762004 11948165 11948146 23002217 22383849 24310371 15378759</pubmed>
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| [[Category:Protein-coding genes]]
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