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| * '''Description:''' phosphoglycerate kinase, glycolytic/ gluconeogenic enzyme, [[universally conserved protein]]<br/><br/>
| | {{#bank: Pgk}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''pgk''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || No
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| |style="background:#ABCDEF;" align="center"| '''Product''' || phosphoglycerate kinase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || enzyme in glycolysis/ gluconeogenesis
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33930 pgk]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=pgk pgk]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 42,0 kDa, 4.77
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1182 bp, 394 amino acids
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tpi]]'', ''[[gapA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU33930 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU33930 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU33930 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:pgk_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pgk_3480197_3481381_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:pgk_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33930]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[ATP synthesis]]}},
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| {{SubtiWiki category|[[carbon core metabolism]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}},
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| {{SubtiWiki category|[[universally conserved proteins]]}},
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| [[most abundant proteins]]
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CggR regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU33930
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| ===Phenotypes of a mutant ===
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| * essential {{PubMed|12682299}}, non-essential according to {{PubMed|23420519}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU33930&redirect=T BSU33930]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cggR-gapA-pgk-tpiA-pgm-eno.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11062]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' ATP + 3-phospho-D-glycerate = ADP + 1,3-bisphosphoglycerate (according to Swiss-Prot)
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| * '''Protein family:''' phosphoglycerate kinase family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:''' Two Substrate Reversible Michaelis-Menten [http://www.ncbi.nlm.nih.gov/pubmed/7154941 PubMed]
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| * '''[[Domains]]:'''
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| ** nucleotide binding domain (ATP) (350–353)
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| ** 2x substrate binding domain (21–23), (59–62)
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| * '''Modification:''' phosphorylation on Ser-183 AND Thr-299 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed]
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| * '''[[Cofactors]]:''' Mg2+ or Mn2+ [http://www.ncbi.nlm.nih.gov/pubmed/7154941 PubMed]
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| * '''Effectors of protein activity:'''
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| ** Inhibited by Co2+, NDP and NMP [http://www.ncbi.nlm.nih.gov/pubmed/7154941 PubMed]
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot), Cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU33930&redirect=T BSU33930]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1PHP 1PHP] (from ''Geobacillus stearothermophilus'')
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P40924 P40924]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU33930]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.2.3 2.7.2.3]
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| === Additional information===
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| * extensive information on the structure and enzymatic properties of Pgk can be found at [http://www.proteopedia.org/wiki/index.php/Phosphoglycerate_Kinase Proteopedia]
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| =Expression and regulation=
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| * '''Operon:'''
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| ** ''[[cggR]]-[[gapA]]-[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]'' {{PubMed|11489127}}
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| ** ''[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]'' {{PubMed|11489127}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pgk_3480197_3481381_-1 pgk] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| ** ''[[cggR]]'': [[SigA]] {{PubMed|11489127}}
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| ** ''[[pgk]]'': [[SigA]] {{PubMed|11489127}}
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| * '''Regulation:'''
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| ** expression activated by glucose (3.3 fold) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
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| ** ''[[cggR]]'': induced by glycolytic substrates [[CggR]] {{PubMed|11489127}}
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| ** ''[[pgk]]'': constitutive {{PubMed|11489127}}
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| * '''Regulatory mechanism:''' transcription repression by [[CggR]] {{PubMed|11489127}}
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| * '''Additional information:'''
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| ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 12006 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 10462 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** GP699 (''pgk''::''cat''), available in [[Jörg Stülke]]'s lab, {{PubMed|23420519}}lab
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| ** GP707 (''pgk''::''erm''), available in [[Jörg Stülke]]'s lab, {{PubMed|23420519}}
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| * '''Expression vector:'''
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| ** pGP1102 (N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
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| ** pGP95 (N-terminal Strep-tag, in [[pGP172]]), available in [[Jörg Stülke]]'s lab
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| ** pGP91 (N-terminal Strep-tag, for [[SPINE]], expression in ''B. subtilis'', in [[pGP380]]), available in [[Jörg Stülke]]'s lab
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| ** pGP1513 (expression in ''B. subtilis'', in [[pBQ200]]), available in [[Jörg Stülke]]'s lab
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| * '''lacZ fusion:''' pGP514 (in [[pAC6]]), a series of promoter deletion variants is also available in [[pAC6]], available in [[Jörg Stülke]]'s lab
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| * '''GFP fusion:'''
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| * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available iin [[Jörg Stülke]]'s lab
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed> 16479537, 12850135 , 17726680, 11489127 ,17505547 , 17218307 7154941 12682299 23420519 15378759</pubmed>
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| [[Category:Protein-coding genes]]
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