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| * '''Description:''' [[response regulator aspartate phosphatase]], anti-activator protein, controls [[ComA]] activity <br/><br/>
| | {{#bank: RapF}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''rapF''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywhJ ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[response regulator aspartate phosphatase]]
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || control of [[ComA]] activity
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37460 rapF]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RapF RapF]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 5.18
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1143 bp, 381 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywhK]]'', ''[[phrF]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37460 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37460 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37460 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:rapF_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rapF_3846001_3847146_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:rapF_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37460]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[genetic competence]]}},
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| {{SubtiWiki category|[[protein modification]]}},
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| {{SubtiWiki category|[[transcription factors and their control]]}},
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| {{SubtiWiki category|[[quorum sensing]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CcpA regulon]]}},
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| {{SubtiWiki regulon|[[ComA regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU37460
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU37460&redirect=T BSU37460]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rapF-phrF.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11968]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' control of [[ComA]] activity {{PubMed|15968044}}
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| * '''Protein family:''' RAP family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:''' binding of [[PhrF]] to [[RapF]] results in a conformational change of [[RapF]] and prevents it from binding [[ComA]] {{PubMed|15968044,22215984}}
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[RapF]]-[[ComA]] {{PubMed|15968044,22215984}}
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| ** [[RapF]]-[[PhrF]] {{PubMed|15968044,22215984}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU37460&redirect=T BSU37460]
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| * '''Structure:'''
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| ** [http://www.rcsb.org/pdb/explore.do?structureId=3ULQ 3ULQ] (structure of the complex between the DNA-binding C-terminal domain of [[ComA]] with [[RapF]]) {{PubMed|22215984}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P71002 P71002]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU37460]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[rapF]]-[[phrF]]'' {{PubMed|11466295}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rapF_3846001_3847146_1 rapF] {{PubMed|22383849}}
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| * '''Sigma factor:'''
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| * '''Regulation:''' repressed by glucose (5-fold) ([[CcpA]]) {{PubMed|12850135}}
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| * '''Regulatory mechanism:'''
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| ** [[CcpA]]: transcription repression {{PubMed|12850135}}
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| ** [[ComA]]: transcription activation {{PubMed|15968044}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>12850135, 15968044 11466295 16091051 22215984</pubmed>
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| [[Category:Protein-coding genes]]
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