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| * '''Description:''' [[protein kinases and phosphatases|protein tyrosine kinase]] <br/><br/>
| | {{#bank: PtkA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''ptkA''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywqD ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[protein kinases and phosphatases|protein tyrosine kinase]]
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || protein phosphorylation
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36250 ptkA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PtkA PtkA]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 25 kDa, 9.628
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 711 bp, 237 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ptpZ]]'', ''[[tkmA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36250 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36250 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36250 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ywqD_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |-
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ptkA_3731822_3732535_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:ptkA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36250]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[biofilm formation]]}},
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| {{SubtiWiki category|[[protein modification]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[AbrB regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU36250
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| ===Phenotypes of a mutant ===
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| * Accumulation of extra chromosome equivalents [http://www.ncbi.nlm.nih.gov/sites/entrez/17367396 PubMed]
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| * Defect in [[biofilm formation]], this involves the kinase activity, but the target protein is unknown {{PubMed|20815827}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU36250&redirect=T BSU36250]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywqCD.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12508]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate (according to Swiss-Prot), autophosphorylation, phosphorylation of [[Ugd]], [[TuaD]], [[Ssb]], [[SsbB]]
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| * '''Protein family:''' BY-kinase, see the [http://bykdb.ibcp.fr/BYKdb/BYKdbHelp Bacterial Protein Tyrosine Kinase Database])
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| * '''Paralogous protein(s):''' [[EpsB]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:''' single BY-kinase domain
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| * '''Modification:''' autophosphorylation at residues Y225, Y227 and Y228 (primary site) {{PubMed|20509597}}, dephosphorylated by [[PtpZ]] {{PubMed|15866923}}
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| * '''[[Cofactors]]:''' ATP
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| * '''Effectors of protein activity:''' [[TkmA]] - transmembrane modulator, activates PtkA autophosphorylation and substrate phosphorylation [http://www.ncbi.nlm.nih.gov/sites/entrez/12970183 PubMed]
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[PtkA]]-[[TkmA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12970183 PubMed]
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| ** [[FatR]]-[[PtkA]] {{PubMed|23939619}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU36250&redirect=T BSU36250]
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| * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2VED 2VED] (CapB, the homolog in ''Staphylococcus aureus'')
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P96716 P96716]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU36250]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[tkmA]]-[[ptkA]]-[[ptpZ]]-[[ugd]]'' {{PubMed|20815827,12970183}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ptkA_3731822_3732535_-1 ptkA] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|20815827}}
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| ** [[DegU]]-P: transcription repression {{PubMed|20815827}}
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| ** [[AbrB]]: transcription repression {{PubMed|20817675}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| ** KO strain created with pMUTIN-2, available from [[Ivan Mijakovic]]
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| ** GP1520 (spc), available in [[ Jörg Stülke]]'s lab
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| ** GP1544 (ermC), available in [[ Jörg Stülke]]'s lab
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| ** GP1587 (cat) , available in [[ Jörg Stülke]]'s lab
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| ** GP1521 ''[[epsB]]'' (aphA3) ''[[ptkA]]'' (spc) double mutant available in [[ Jörg Stülke]]'s lab
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| ** GP1529 ''[[tkmA]]-[[ptkA]]''::spc available in [[ Jörg Stülke]]'s lab
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| ** GP1610 (''[[ptkA]]-[[ptpZ]]'', spc), available in [[ Jörg Stülke]]'s lab
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| * '''Expression vector:''' pQE-30, N-terminally 6xHis-tagged, available from [[Ivan Mijakovic]]
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| * '''lacZ fusion:''' in a KO strain created with pMUTIN-2, available from [[Ivan Mijakovic]]
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| * '''GFP fusion:''' CFP-fusion, available from [[Ivan Mijakovic]]
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| * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[ Jörg Stülke]]'s lab
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Ivan Mijakovic]], Thiverval-Grignon, France
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 20497498 </pubmed>
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| ==Original publications==
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| <pubmed> 12970183, 15741737, 15866923, 17367396, 19258708 18547145 20497499 20509597 20815827 20817675 23939619 24493247 </pubmed>
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| [[Category:Protein-coding genes]]
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