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| * '''Description:''' transcriptional activator of competence development and sporulation genes, represses [[SigD]]-dependent flagellar genes, antagonist of [[SlrA]] and [[SinR]], has LexA-like autocleavage activity<br/><br/>
| | {{#bank: SlrR}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''slrR''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yveJ, slr''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || transcription regulator, [[SlrA]] antagonist
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of initiation of [[biofilm formation]] and of autolysis
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34380 slrR]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=SlrR SlrR]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=slrR slrR]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 9.63
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 456 bp, 152 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[epsA]]'', ''[[pnbA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU34380 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU34380 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU34380 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:slr_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=slrR_3530101_3530559_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:slrR_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34380]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[transcription factors and their control]]}},
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| {{SubtiWiki category|[[transition state regulators]]}},
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| {{SubtiWiki category|[[biofilm formation]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[Abh regulon]]}},
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| {{SubtiWiki regulon|[[AbrB regulon]]}},
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| {{SubtiWiki regulon|[[SinR regulon]]}}
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|
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| =The [[SlrR regulon]]:=
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| * ''[[lytA]]-[[lytB]]-[[lytC]]'', ''[[lytF]]''
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU34380
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| ===Phenotypes of a mutant ===
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| ** smooth colonies on MsGG medium, no [[biofilm formation]] {{PubMed|22113911}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU34380&redirect=T BSU34380]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11858]
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| === Additional information===
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| * overexpression of ''slrR'' suppresses the phenotype of a [[ymdB]] mutant {{PubMed|21856853}}
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** [[SlrR]] binds to and inhibits the activity of [[SlrA]], [[SlrA]] indirectly stimulates the synthesis of [[SlrR]] by interacting with [[SinR]]. [[SlrR]] can bind to [[SinR]] and [[SinR]] directly represses the transcription of [[SlrR]]. [[SlrR]] indirectly derepresses its own gene. The heterocomplex of [[SlrR]]-[[SinR]] is a repressor of autolysin and motility genes and inhibits the repressor function of [[SinR]]. {{PubMed|19788541,20923420,20351052}}
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| ** repression of transcription of ''[[lytA]]-[[lytB]]-[[lytC]]'' and ''[[lytF]]'' {{PubMed|20351052}}
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| ** autocleavage {{PubMed|20923420}}
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| * '''Protein family:'''
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| * '''Paralogous protein(s):''' [[SinR]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| ** subject to self-cleavage via a LexA-like autopeptidase, this involves [[ClpC]] {{PubMed|20923420}}
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:''' interaction with [[SinR]] triggers binding of SlrR to the promoters of ''[[lytA]]-[[lytB]]-[[lytC]]'' and ''[[lytF]]'', resulting in their repression {{PubMed|20351052}}
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[SlrA]]-[[SlrR]] {{PubMed|19788541}}
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| ** [[SlrR]]-[[SinR]] {{PubMed|20351052}}, K(D) 47.5 nM {{PubMed|23430750}}, [[Veg]] may inhibit this interaction {{PubMed|23378512}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU34380&redirect=T BSU34380]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P71049 P71049]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU34380]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[slrR]]'' {{PubMed|21815947}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=slrR_3530101_3530559_1 slrR] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| ** [[Abh]]: transcription activation {{PubMed|19767430}}
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| ** [[SinR]]: transcription repression {{PubMed|19788541}}
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| ** [[AbrB]]: transcription repression {{PubMed|20817675}}
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| * '''Additional information:'''
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| ** induction by sequestration of [[SinR]] by [[SinI]], [[SlrA]] {{PubMed|15661000,19788541}} or by SlrR itself {{PubMed|20351052}}
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| ** the ''[[slrR]]'' gene is not expressed in a ''[[ymdB]]'' mutant {{PubMed|21856853}}
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| ** the amount of the mRNA is substantially decreased upon depletion of [[Rny|RNase Y]] (this is likely due to the increased stability of the ''[[sinR]]'' mRNA) {{PubMed|21815947}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** GP955 (''slrR''-''[[pnbA]]''::''cat''), available in [[Jörg Stülke]]'s lab
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in [[Stülke]] lab
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 20541494 23353768 </pubmed>
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| ==Original publications==
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| <pubmed>23378512 23430750 21856853 22329926,22113911,20817675 18430133,18647168, 19767430, 19788541 20351052 20923420 24256735 </pubmed>
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| [[Category:Protein-coding genes]]
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