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| * '''Description:''' fructoselysine-6-P-glycosidase <br/><br/>
| | {{#bank: FrlB}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''frlB''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yurP ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || fructoselysine-6-P-glycosidase
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || metabolism of aminoacylated fructose
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU32610 frlB]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=frlB frlB]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 5.442
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 984 bp, 328 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[frlO]]'', ''[[yurQ]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU32610 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU32610 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU32610 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:yurP_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=frlB_3351110_3352096_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:frlB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU32610]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[utilization of specific carbon sources]]}},
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| {{SubtiWiki category|[[utilization of nitrogen sources other than amino acids]]}},
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| {{SubtiWiki category|[[membrane proteins]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}},
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| [[most abundant proteins]]
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CodY regulon]]}}, {{SubtiWiki regulon|[[FrlR regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU32610
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU32610&redirect=T BSU32610]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yurRQPONML.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG14002]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| ** phosphorylated on Arg-48 {{PubMed|22517742}}
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:''' membrane associated [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU32610&redirect=T BSU32610]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3EUA 3EUA]
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O32157 O32157]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU32610]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| ** ''[[frlB]]-[[frlO]]-[[frlN]]-[[frlM]]-[[frlD]]'' {{PubMed|23175651,21347729}}
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| ** ''[[frlB]]-[[frlO]]'' {{PubMed|23175651}}
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| ** ''[[frlB]]'' {{PubMed|23175651}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=frlB_3351110_3352096_-1 frlB] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| **'' [[frlB]]'': repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
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| ** ''[[frlB]]'': induced in the presence of fructosamine ([[FrlR]]) {{PubMed|21397556}}
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| * '''Regulatory mechanism:'''
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| ** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed1], [http://www.ncbi.nlm.nih.gov/sites/entrez/18083814 PubMed2]
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| ** [[FrlR]]: transcription repression {{PubMed|21397556}}
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| * '''Additional information:'''
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| ** the mRNA is substantially stabilized upon depletion of [[Rny|RNase Y]] {{PubMed|21815947}}
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| ** the ''[[frlB]]-[[frlO]]-[[frlN]]-[[frlM]]-[[frlD]]'' operon is not expressed in a ''[[cshA]]'' mutant {{PubMed|23175651}}
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| ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>22517742 23175651 21815947 15556630,18083814,12618455, 21347729, 18763711, 21398478 15378759</pubmed>
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| [[Category:Protein-coding genes]]
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