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| * '''Description:''' tryptophan operon RNA-binding attenuation protein (TRAP), controls the [[RNA switch]] in front of genes involved in biosynthesis and acquisition of tryptophan <br/><br/>
| | {{#bank: MtrB}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''mtrB''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || tryptophan operon RNA-binding attenuation protein (TRAP)
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of tryptophan biosynthesis<br/>(and translation) attenuation in the trp operon;<br/>repression of the folate operon
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22770 mtrB]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MtrB TRAP]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=mtrB mtrB]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 8 kDa, 7.333
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 225 bp, 75 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[hepS]]'', ''[[folE]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU22770 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU22770 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU22770 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:mtrB_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mtrB_2384534_2384761_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:mtrB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22770]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}},
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| {{SubtiWiki category|[[transcription factors and their control]]}},
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| {{SubtiWiki category|[[RNA binding regulators]]}}
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| = This gene is a member of the following [[regulons]] =
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| = The [[TRAP regulon]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU22770
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU22770&redirect=T BSU22770]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mtrAB.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10278]
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| === Additional information===
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| * mutations in ''[[mtrB]]'', ''[[sigB]]'', ''[[rpoB]]'', and ''[[rpoC]]'' allow ''B. subtilis'' to grow with 4-fluorotryptophan rather than with tryptophan as a canonical amino acid of the genetic code {{PubMed|24572018}}
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' prpD family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| ** binding of tryptophan results RNA binding and thus in transcription termination
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| * '''[[SubtInteract|Interactions]]:'''
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| ** forms 11-mers
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| ** [[MtrB|TRAP]]<sub>11</sub>-[[RtpA]]<sub>3</sub> {{PubMed|24682818,11557884}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU22770&redirect=T BSU22770]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3AQD 3AQD], [http://www.rcsb.org/pdb/explore.do?structureId=1WAP 1WAP] (complex with L-tryptophan), [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2ZP9 2ZP9] (complex with [[RtpA]])
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P19466 P19466]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU22770]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[folE]]-[[mtrB]]'' {{PubMed|2123343}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mtrB_2384534_2384761_-1 mtrB] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|2123343}}
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>19385727, 16285852, 15063849 </pubmed>
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| ==Original Publications==
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| <pubmed> 22970197 19033375, 17881743 10660627 10714985 12963367 14702295 16879415 17114058 17114263 11805104,7525975,10735881,15262953,14976255,8647825,18334255,10499579,7715723,8932308,11214184,15743934,7515880,9242649,7759487,15050822,14687568,9245598,9765233,11914485,15099736,7678334,8643393,15588817,16738132,1551827,17555767,7544009,11786553,11566976,2123343,11557884,12133840,16784236,10369778,7715723 10499579 11566976 7515880, 21097886 21984911 24505391 24572018 24682818 </pubmed>
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| [[Category:Protein-coding genes]]
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