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| * '''Description:''' sporulation-specific diadenylate cyclase, synthesis of c-di-AMP<br/><br/>
| | {{#bank: CdaS}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''cdaS''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yojJ ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || sporulation-specific diadenylate cyclase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of c-di-AMP
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU19430 cdaS]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 22 kDa, 5.367
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 621 bp, 207 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yojK]]'', ''[[yojI]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU19430 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU19430 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU19430 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:yojJ_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yojJ_2118504_2119127_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:yojJ_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU19430]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[metabolism of signalling nucleotides]]}},
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| {{SubtiWiki category|[[sporulation proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[SigG regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU19430
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU19430&redirect=T BSU19430]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13562]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** synthesis of c-di-AMP from two molecules of ATP {{PubMed|23192352}}
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| * '''Protein family:'''
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| * '''Paralogous protein(s):''' [[CdaA]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| ** contains a [[DAC domain]] involved in the synthesis of c-di-AMP {{PubMed|21566650}}
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU19430&redirect=T BSU19430]
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| * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=2FB5 2FB5] (the protein of ''B. cereus'', 48% identity, 81% similarity)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O31854 O31854]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU19430]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[cdaS]]'' {{PubMed|22383849}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yojJ_2118504_2119127_-1 cdaS] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigG]] {{PubMed|22383849}}
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| * '''Regulation:'''
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| ** expressed late during sporulation in the forespore ([[SigG]]) {{PubMed|22383849}}
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| ** GP983 (''[[cdaS]]''::''ermC''), available in [[Jörg Stülke]]'s lab
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| ** GP1360 (''[[cdaS]]''::''spc''), available in [[Jörg Stülke]]'s lab
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| * '''Expression vector:'''
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| ** IPTG inducible expression of '''''B. cereus''''' Strep-''cdaS'' in ''E. coli'': pGP2593 (in [[pGP172]]), available in [[Jörg Stülke]]'s lab
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''His-tag fusion:'''
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| **pGP2591 10xHis-''cdaS'' under the control of native promoter (based on [[pAC7]]), available in [[Jörg Stülke]]'s lab
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| **pGP2592 10xHis-''cdaS-L44F'' under the control of native promoter (based on [[pAC7]]), available in [[Jörg Stülke]]'s lab
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 18714086</pubmed>
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| ==Original publications==
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| <pubmed>21566650, 23192352 22383849</pubmed>
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| [[Category:Protein-coding genes]]
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