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| * '''Description:''' ATP-dependent DNA translocase, resolution of chromosomal dimers after DNA replication, transports the forespore chromosome across the sporulation septum <br/><br/>
| | {{#bank: SpoIIIE}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''spoIIIE''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || ATP-dependent DNA translocase required for chromosome
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| partitioning through the septum into the forespore
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || chromosome partition during sporulation
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16800 spoIIIE]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 86 kDa, 6.757
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2361 bp, 787 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylzJ]]'', ''[[ymfC]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU16800 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU16800 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU16800 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:spoIIIE_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spoIIIE_1752272_1754641_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:spoIIIE_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16800]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[DNA condensation/ segregation]]}},
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| {{SubtiWiki category|[[sporulation/ other]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU16800
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16800&redirect=T BSU16800]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spoIIIE.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10763]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** transports the forespore chromosome across the sporulation septum {{PubMed|20516200}}
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| ** translocates septum-entrapped DNA only when septum closure precedes complete segregation of chromosomes {{PubMed|19818024}}
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| ** the two DNA translocases [[SftA]] and [[SpoIIIE]] synergistically affect chromosome dimer resolution presumably by positioning the dif sites in close proximity, before or after completion of cell division, respectively {{PubMed|21239579}}
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| ** binds DNA sequences named SRS for SpoIIIE recognition sequence, GAGAAGGG {{PubMed|18391964}}
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| * '''Protein family:'''
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| * '''Paralogous protein(s):''' [[SftA]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| ** N-terminal transmembrane domain (4 helices) {{PubMed|24297254}}
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| ** 134 aa linker {{PubMed|24297254}}
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| ** 512 aa motor domain at the C-terminus {{PubMed|24297254}}
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** assembles into ∼45 nm complexes {{PubMed|23667326}}
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| * '''[[Localization]]:'''
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| ** transmembrane domain mediates dynamic localization to active division sites {{PubMed|20516200}}
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| ** complexes are recruited to nascent sites of septation, and are subsequently escorted by the constriction machinery to the center of [[sporulation]] and division septa {{PubMed|23667326}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16800&redirect=T BSU16800]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P21458 P21458]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16800]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''spoIIIE'' {{PubMed|3129532}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spoIIIE_1752272_1754641_1 spoIIIE] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|2507870}}
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 22047950 22934648 23400100 </pubmed>
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| ==Original publications==
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| <pubmed> 18160039, 18593879, 3129532 2507870 18593879 23559069 9150232,2492502, 11062134,9155041, 14680637,7797072, 7567988, 2507870,8160014,9515703, 19818024 19788545 20298190 20516200 21239579 18391964 23667326 11134515 24297254</pubmed>
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| [[Category:Protein-coding genes]]
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