|
|
| (3 intermediate revisions by 2 users not shown) |
| Line 1: |
Line 1: |
| * '''Description:''' aspartate-semialdehyde dehydrogenase <br/><br/>
| | {{#bank: Asd}} |
|
| |
|
| {| align="right" border="1" cellpadding="2"
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''asd''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || aspartate-semialdehyde dehydrogenase
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of threonine, lysine, dipicolic acid, peptidoglycan
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16750 asd]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=Asd Asd]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=asd asd]'''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 4.971
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1038 bp, 346 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spoVFB]]'', ''[[dapG]]''
| |
| |-
| |
| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU16750 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU16750 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU16750 DNA_with_flanks]
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:asd_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=asd_1745991_1747031_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:asd_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16750]]
| |
| |-
| |
| |}
| |
|
| |
| __TOC__
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/>
| |
|
| |
| = [[Categories]] containing this gene/protein =
| |
| {{SubtiWiki category|[[cell wall synthesis]]}},
| |
| {{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}},
| |
| {{SubtiWiki category|[[Biosynthesis of cell wall components]]}},
| |
| {{SubtiWiki category|[[sporulation proteins]]}},
| |
| {{SubtiWiki category|[[essential genes]]}},
| |
| {{SubtiWiki category|[[phosphoproteins]]}}
| |
|
| |
| = This gene is a member of the following [[regulons]] =
| |
| {{SubtiWiki regulon|[[SigK regulon]]}}
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU16750
| |
|
| |
| ===Phenotypes of a mutant ===
| |
|
| |
| essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
| |
|
| |
| === Database entries ===
| |
| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16750&redirect=T BSU16750]
| |
|
| |
| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spoVFAB-asd-dapG-dapA.html]
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10783]
| |
|
| |
| === Additional information===
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:''' L-aspartate 4-semialdehyde + phosphate + NADP<sup>+</sup> = L-4-aspartyl phosphate + NADPH (according to Swiss-Prot)
| |
|
| |
| * '''Protein family:''' aspartate-semialdehyde dehydrogenase family (according to Swiss-Prot)
| |
|
| |
| * '''Paralogous protein(s):'''
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''[[Domains]]:'''
| |
|
| |
| * '''Modification:''' phosphorylation on Ser-98 AND Tyr-146 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]
| |
|
| |
| * '''[[Cofactors]]:'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''[[SubtInteract|Interactions]]:'''
| |
|
| |
| * '''[[Localization]]:'''
| |
|
| |
| === Database entries ===
| |
| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16750&redirect=T BSU16750]
| |
|
| |
| * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2GYY 2GYY] (from ''Streptococcus pneumoniae'', 52% identity, 65% similarity) {{PubMed|16895909}}
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/Q04797 Q04797]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16750]
| |
|
| |
| * '''E.C. number:''' [http://www.expasy.org/enzyme/1.2.1.11 1.2.1.11]
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
|
| |
| * '''Operon:'''
| |
| ** ''[[spoVFA]]-[[spoVFB]]-[[asd]]-[[dapG]]-[[dapA]]'' {{PubMed|8098035}}
| |
| ** ''[[asd]]-[[dapG]]-[[dapA]]'' {{PubMed|8098035}}
| |
|
| |
| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=asd_1745991_1747031_1 asd] {{PubMed|22383849}}
| |
|
| |
| * '''[[Sigma factor]]:'''
| |
| ** ''[[spoVFA]]'': [[SigK]] {{PubMed|15699190,8098035}}
| |
| ** ''[[asd]]'': [[SigA]] {{PubMed|8098035}}
| |
|
| |
| * '''Regulation:'''
| |
|
| |
| * '''Regulatory mechanism:'''
| |
|
| |
| * '''Additional information:'''
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:'''
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
| <pubmed>8345520,,15699190 8098035,,17218307, </pubmed>
| |
| [[Category:Protein-coding genes]]
| |
