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| * '''Description:''' major extracellular alkaline protease <br/><br/>
| | {{#bank: AprE}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''aprE''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''sprE ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || extracellular alkaline serine protease (subtilisin E))
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || protein degradation
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU10300 aprE]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=AprE AprE]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 39 kDa, 9.342
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1143 bp, 381 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhfN]]'', ''[[yhfO]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU10300 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU10300 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU10300 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:aprE_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=aprE_1104423_1105568_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:aprE_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU10300]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[utilization of nitrogen sources other than amino acids]]}},
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| {{SubtiWiki category|[[proteolysis]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[AbrB regulon]]}},
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| {{SubtiWiki regulon|[[ScoC regulon]]}},
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| {{SubtiWiki regulon|[[SinR regulon]]}}
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|
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU10300
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU10300&redirect=T BSU10300]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/aprE.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10190]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1 (according to Swiss-Prot)
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| * '''Protein family:''' peptidase S8 family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[AprE]]-[[PaiA]] {{PubMed|11325965}}
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| * '''[[Localization]]:''' secreted (according to Swiss-Prot), extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU10300&redirect=T BSU10300]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1SBC 1SBC]
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P04189 P04189]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU10300]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' aprE (according to [http://dbtbs.hgc.jp/COG/prom/aprE.html DBTBS])
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=aprE_1104423_1105568_-1 aprE] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|2447063}}
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| * '''Regulation:'''
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| ** repressed by [[SinR]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1898931 PubMed]
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| ** repressed during exponential growth ([[ScoC]], [[AbrB]]) {{PubMed|1906467,2504584}}
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| * '''Regulatory mechanism:'''
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| ** [[SinR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/1898931 PubMed]
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| ** [[ScoC]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/1906467 PubMed]
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| ** [[AbrB]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/2504584 PubMed]
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| * '''Additional information:''' the mRNA is extremely stable (more than 25 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/11101663 PubMed]
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| * '''Additional information:''' The pAPNC213 vector published by Morimoto et al. (2002) is an insertion plasmid for IPTG inducible constructs that can be integrated into the chromosomal aprE locus by replacing the aprE open reading frame via double homologous recombination. [http://www.ncbi.nlm.nih.gov/sites/entrez/12427945 PubMed]
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>20735481 </pubmed>
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| ==Original publications==
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| <pubmed> 19710937 3082852,2447063,2447062,19251843,18414485, 6322190,12884008,15126467,1898931,1906467,2504584, 18957862 11101663 12055299, 3131303 1906467 15598897 11325965 14688235 21099137 22745669 23563549 23563567 24115457 </pubmed>
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| [[Category:Protein-coding genes]]
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