|
|
| Line 1: |
Line 1: |
| * '''Description:''' pectate lyase C <br/><br/>
| | {{#bank: Pel}} |
| {| align="right" border="1" cellpadding="2" | |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''pel''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || pectate lyase C
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || degradation of polygalacturonic acid
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU07560 pel]
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 8.421
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1260 bp, 420 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yflT]]'', ''[[yflS]]''
| |
| |-
| |
| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU07560 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU07560 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU07560 DNA_with_flanks]
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:pel_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pel_827993_829255_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:pel_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU07560]]
| |
| |-
| |
| |}
| |
|
| |
|
| __TOC__
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
|
| |
|
| |
| <br/><br/>
| |
|
| |
| = [[Categories]] containing this gene/protein =
| |
| {{SubtiWiki category|[[utilization of specific carbon sources]]}}
| |
|
| |
| = This gene is a member of the following [[regulons]] =
| |
| {{SubtiWiki regulon|[[CcpA regulon]]}},
| |
| {{SubtiWiki regulon|[[ComA regulon]]}},
| |
| {{SubtiWiki regulon|[[TnrA regulon]]}}
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU07560
| |
|
| |
| ===Phenotypes of a mutant ===
| |
|
| |
| === Database entries ===
| |
| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU07560&redirect=T BSU07560]
| |
|
| |
| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pel.html]
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10840]
| |
|
| |
| === Additional information===
| |
|
| |
|
| |
|
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:''' Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends (according to Swiss-Prot)
| |
|
| |
| * '''Protein family:''' polysaccharide lyase 1 family (according to Swiss-Prot)
| |
|
| |
| * '''Paralogous protein(s):'''
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''Domains:'''
| |
|
| |
| * '''Modification:'''
| |
|
| |
| * '''Cofactor(s):'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''[[SubtInteract|Interactions]]:'''
| |
|
| |
| * '''[[Localization]]:'''
| |
| ** extracellular (signal peptide), major constituent of the secretome [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
| |
|
| |
| === Database entries ===
| |
| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU07560&redirect=T BSU07560]
| |
|
| |
| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2O1D 2O1D] (bound to trisaccharide), [http://www.rcsb.org/pdb/explore.do?structureId=1BN8 1BN8]
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/P39116 P39116]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU07560]
| |
|
| |
| * '''E.C. number:''' [http://www.expasy.org/enzyme/4.2.2.2 4.2.2.2]
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
|
| |
| * '''Operon:''' ''pel'' {{PubMed|12823818}}
| |
|
| |
| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pel_827993_829255_1 pel] {{PubMed|22383849}}
| |
|
| |
| * '''Sigma factor:'''
| |
|
| |
| * '''Regulation:'''
| |
| ** repressed by glucose (3.8-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
| |
| ** repressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) {{PubMed|12823818}}
| |
| ** expressed at high cell density ([[ComA]]) {{PubMed|16091051}}
| |
|
| |
| * '''Regulatory mechanism:'''
| |
| ** [[ComA]]: transcription activation ([[ComA]]) {{PubMed|16091051}}
| |
| ** [[CcpA]]: transcription repression
| |
| ** [[TnrA]]: transcription repression {{PubMed|12823818}}
| |
|
| |
| * '''Additional information:'''
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:'''
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
| ==Reviews==
| |
| <pubmed>20735481 </pubmed>
| |
| ==Original publications==
| |
| <pubmed>8262178 ,16091051,12823818,18957862 12850135, </pubmed>
| |
|
| |
| [[Category:Protein-coding genes]]
| |
