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| * '''Description:''' high affinity proline [[permease]] <br/><br/>
| | {{#bank: PutP}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''putP''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ycgO ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || high affinity proline [[permease]]
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || proline uptake
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03220 putP]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 48 kDa, 8.619
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1347 bp, 449 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[putC]]'', ''[[putR]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU03220 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU03220 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU03220 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ycgO_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ycgO_347150_348571_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:putP_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03220]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[transporters/ other]]}},
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| {{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}},
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| {{SubtiWiki category|[[utilization of amino acids]]}}
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| {{SubtiWiki category|[[membrane proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[PutR regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU03220
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| ===Phenotypes of a mutant ===
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| * no growth with proline as single source of carbon or nitrogen {{PubMed|21840319}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU03220&redirect=T BSU03220]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12013]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** uptake of proline coupled to the uptake of sodium ions
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| * '''Protein family:''' sodium solute symporter family (SSS; TC2A.21)
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| * '''Paralogous protein(s):''' [[OpuE]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| ** K(M) for proline: 8 +/- 2 µM {{PubMed|22139509}}
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:''' cell membrane (according to Swiss-Prot)
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU03220&redirect=T BSU03220]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P94392 P94392]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU03220]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| ** ''[[putB]]-[[putC]]-[[putP]]'' {{PubMed|21840319,22139509}}
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| ** ''[[putP]]'' (internal promoter) {{PubMed|21840319}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ycgO_347150_348571_1 putP] {{PubMed|22383849}}
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| * '''Sigma factor:'''
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| ** ''[[putB]]'': [[SigA]] {{PubMed|21840319,21964733,22139509}}
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| * '''Regulation:'''
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| ** induced in the presence of proline ([[PutR]]) {{PubMed|21840319,22139509}}
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| * '''Regulatory mechanism:'''
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| ** [[PutR]]: transcription activation {{PubMed|21840319,21964733,22139509}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| ** available in [[Erhard Bremer]]'s lab {{PubMed|24142252}}
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed> 21840319 21964733,22139509 24142252 </pubmed>
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| [[Category:Protein-coding genes]]
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