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| * '''Description:''' phosphotransacetylase <br/><br/>
| | {{#bank: Pta}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''pta''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipa-88d, ywfJ ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || phosphotransacetylase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || overflow metabolism
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37660 pta]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=pta pta]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 4.649
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 969 bp, 323 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cysL]]'', ''[[ywfI]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37660 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37660 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37660 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:pta_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pta_3865355_3866326_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:pta_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37660]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[carbon core metabolism]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CcpA regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU37660
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pta.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10634]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Acetyl-CoA + phosphate = CoA + acetyl phosphate (according to Swiss-Prot)
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| * '''Protein family:''' phosphate acetyltransferase and butyryltransferase family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:''' phosphorylation on (Ser-123 OR Thr-128 OR Ser-129) [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1XCO 1XCO] (complex with acetylphosphate), [http://www.rcsb.org/pdb/explore.do?structureId=1TD9 1TD9]
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P39646 P39646]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU37660]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.1.8 2.3.1.8]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''pta'' {{PubMed|10423526}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pta_3865355_3866326_-1 pta] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] {{PubMed|10423526}}
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| * '''Regulation:'''
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| ** expression activated by glucose (2.6 fold) (CcpA) {{PubMed|12850135,10559153}}
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| * '''Regulatory mechanism:'''
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| ** [[CcpA]]: transcription activation {{PubMed|12850135,10559153}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:''' GP668 (aphA3), available in the [[Stülke]] lab
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 15755952</pubmed>
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| ==Original publications==
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| '''Additional publications:''' {{PubMed|22900538}}
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| <pubmed>10559153, 12850135 17218307 10559153 10423526, 16283428 23054816</pubmed>
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| [[Category:Protein-coding genes]]
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