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| * '''Description:''' acetolactate decarboxylase <br/><br/>
| | {{#bank: AlsD}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''alsD''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || acetolactate decarboxylase)
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| |style="background:#ABCDEF;" align="center"|'''Function''' || overflow metabolism
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36000 alsD]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=alsD alsD]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 4.603
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 765 bp, 255 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywrO]]'', ''[[alsS]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36000 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36000 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36000 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:alsD_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=alsD_3708799_3709566_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:alsD_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36000]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[carbon core metabolism]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[AlsR regulon]]}},
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| {{SubtiWiki regulon|[[Rex regulon]]}},
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| {{SubtiWiki regulon|[[stringent response]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU36000
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/alsSD.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10472]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' (2S)-2-hydroxy-2-methyl-3-oxobutanoate = (3R)-3-hydroxybutan-2-one + CO<sub>2</sub> (according to Swiss-Prot)
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| * '''Protein family:''' alpha-acetolactate decarboxylase family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:''' phosphorylated on ser/ thr/ tyr [http://www.ncbi.nlm.nih.gov/pubmed/16493705 PubMed], ''in vitro'' phosphorylated by [[PrkC]] on Ser-88 {{PubMed|20389117}}
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/Q04777 Q04777]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU36000]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/4.1.1.5 4.1.1.5]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[alsS]]-[[alsD]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/7685336 PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=alsD_3708799_3709566_-1 alsD] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/7685336 PubMed]
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| * '''Regulation:'''
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| ** induction by acetate ([[AlsR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/7685336 PubMed]
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| ** repressed as long as terminal electron acceptors are available for respiration ([[Rex]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/16428414 PubMed]
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| ** subject to positive stringent control upon lysine starvation {{PubMed|20081037}}
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| Note: since acetate formation requires ''[[ackA]]'' activation by [[CcpA]] there is an indirect effect of [[CcpA]] on the ''alsSD'' operon: the operon is not expressed in ''[[ccpA]]'' mutants
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| * '''Regulatory mechanism:'''
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| ** stringent response: due to presence of adenines at +1 and +2 positions of the transcript {{PubMed|20081037}}
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| ** [[AlsR]]: transcription activation in the presence of acetate [http://www.ncbi.nlm.nih.gov/sites/entrez/7685336 PubMed]
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| ** [[Rex]]: transcription repression if the ratio NADH2/NAD is high [http://www.ncbi.nlm.nih.gov/sites/entrez/16428414 PubMed]
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| ** for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]]: pGP822, available in [[Stülke]] lab
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| '''Additional publications:''' {{PubMed|22178965}}
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| <pubmed>19087206,10986270,,16428414,16493705 7685336 16428414, 20081037 20389117 </pubmed>
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| [[Category:Protein-coding genes]]
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