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| * '''Description:''' ATP-dependent deoxyribonuclease (subunit A) <br/><br/>
| | {{#bank: AddA}} |
| {| align="right" border="1" cellpadding="2" | |
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''addA''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''recE5 ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || ATP-dependent deoxyribonuclease (subunit A))
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| |style="background:#ABCDEF;" align="center"|'''Function''' || [[DNA repair/ recombination]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU10630 addA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=AddA AddA]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 140 kDa, 5.127
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 3696 bp, 1232 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[addB]]'', ''[[sbcD]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU10630 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU10630 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU10630 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:addA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=addA_1139807_1143505_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:addA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU10630]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[DNA repair/ recombination]]}},
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| {{SubtiWiki category|[[genetic competence]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[ComK regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU10630
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/addBA.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10466]
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| === Additional information===
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| * A mutation was found in this gene after evolution under relaxed selection for sporulation {{PubMed|21821766}}
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** the enzyme is functional as a heterodimer of the [[AddA]] and [[AddB]] subunits, that it is a rapid and processive DNA helicase, and that it catalyses DNA unwinding using one single-stranded DNA motor of 3'→5' polarity located in the [[AddA]] subunit {{PubMed|21071401}}
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| ** the [[AddB]] subunit contains a second putative ATP-binding pocket, but this does not contribute to the observed helicase activity and may instead be involved in the recognition of recombination hotspot sequences {{PubMed|21071401}}
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| * '''Protein family:''' uvrD-like helicase C-terminal domain (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[AddA]]-[[AddB]] {{PubMed|10756102,21071401}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=3U4Q 3U4Q] (the [[AddA]]-[[AddB]]-DNA complex) {{PubMed|22307084}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P23478 P23478]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU10630]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[addB]]-[[addA]]-[[sbcD]]-[[sbcC]]-[[hlpB]]'' {{PubMed|22383849}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=addA_1139807_1143505_1 addA] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] {{PubMed|7746142}}
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| * '''Regulation:'''
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| ** positive control by [[ComK]] {{PubMed|7746142}}
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:''' GP1106 (addAB, spc), available in [[Stülke]] lab
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| * [[Mark Dillingham]], Bristol, U.K. ([http://www.bris.ac.uk/biochemistry/research/md.html homepage])
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 23202527, 20116346 22933559</pubmed>
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| ==Original publications==
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| '''Additional publications:''' {{PubMed|21071401}}
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| <pubmed>,8387145,15610857,7746142, 19129187 15009890 10756102 9781875 16632468 17570399 20350930 21809208 21821766 22307084 22383849</pubmed>
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| [[Category:Protein-coding genes]]
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