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| * '''Description:''' spore photoproduct lyase <br/><br/>
| | {{#bank: SplB}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''splB''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''spl ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || spore photoproduct lyase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || protection of spore DNA against photodamage
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13930 splB]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 39 kDa, 8.524
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1026 bp, 342 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[splA]]'', ''[[ykwB]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU13930 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU13930 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU13930 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:splB_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=splB_1461770_1462798_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:splB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13930]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[DNA repair/ recombination]]}},
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| {{SubtiWiki category|[[sporulation proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[SigG regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU13930
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/splAB.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10203]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** repairs a special thymine dimer (5-thyminyl-5,6-dihydrothymine), which is commonly called spore photoproduct at the early germination phase
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| ** contains a [4Fe-4S] cluster, uses the [4Fe-4S] cluster to reduce SAM
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=4FHC 4FHC] (SplB from ''Geobacillus thermodenitrificans'', 72% identity, 90% similarity) {{PubMed|22761404}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P37956 P37956]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU13930]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[splA]]-[[splB]]'' {{PubMed|8021181}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=splB_1461770_1462798_1 splB] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigG]] {{PubMed|15699190,8021181}}
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| * '''Regulation:''' expressed during sporulation in the forespore ([[SigG]]) {{PubMed|16497325}}
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| * '''Regulatory mechanism:'''
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| ** negative autoregulation by [[SplA]] {{PubMed|8021181}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:''' BP130 (del ''splB''::''spc''), available in [[Fabian Commichau]]'s lab
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 23164663 </pubmed>
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| ==Original publications==
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| <pubmed> 15699190,8021181 21671623 22197590 23607538 23799365 22761404 </pubmed>
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| [[Category:Protein-coding genes]]
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