|
|
| Line 1: |
Line 1: |
| * '''Description:''' tRNA nucleotidyltransferase, maturation of the single-copy tRNACys, which lacks an encoded CCA 3' end <br/><br/>
| | {{#bank: Cca}} |
|
| |
|
| {| align="right" border="1" cellpadding="2"
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''cca''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''papS, ypjI ''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || tRNA nucleotidyltransferase
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || tRNA modification
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22450 cca]
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 8.041
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1191 bp, 397 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[birA]]'', ''[[bshA]]''
| |
| |-
| |
| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU22450 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU22450 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU22450 DNA_with_flanks]
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:cca_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cca_2355880_2357073_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:cca_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22450]]
| |
| |-
| |
| |}
| |
|
| |
| __TOC__
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/>
| |
|
| |
| = [[Categories]] containing this gene/protein =
| |
| {{SubtiWiki category|[[translation]]}},
| |
| {{SubtiWiki category|[[essential genes]]}}
| |
|
| |
| = This gene is a member of the following [[regulons]] =
| |
| {{SubtiWiki regulon|[[Spx regulon]]}}
| |
|
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU22450
| |
|
| |
| ===Phenotypes of a mutant ===
| |
|
| |
| essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''DBTBS entry:''' no entry
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11208]
| |
|
| |
| === Additional information===
| |
|
| |
|
| |
|
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:''' ATP + tRNA(n) = diphosphate + tRNA(n+1) (according to Swiss-Prot)
| |
|
| |
| * '''Protein family:''' the protein is similar to the ''E. coli'' poly(A) polymerase
| |
|
| |
| * '''Paralogous protein(s):'''
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''Domains:'''
| |
|
| |
| * '''Modification:'''
| |
|
| |
| * '''Cofactor(s):'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''[[SubtInteract|Interactions]]:'''
| |
|
| |
| * '''[[Localization]]:'''
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1MIV 1MIV] (Geobacillus stearothermophilus 44% identity)
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/P42977 P42977]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU22450]
| |
|
| |
| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.7.25 2.7.7.25]
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
|
| |
| * '''Operon:''' ''[[ypjD]]-[[dapB]]-[[mgsA]]-[[bshB1]]-[[bshA]]-[[cca]]-[[birA]]'' {{PubMed|20308541}}
| |
|
| |
| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cca_2355880_2357073_-1 cca] {{PubMed|22383849}}
| |
|
| |
| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|23894131}}
| |
|
| |
| * '''Regulation:'''
| |
| ** induced by diamide stess (thiol depletion) ([[Spx]]) {{PubMed|23894131}}
| |
|
| |
| * '''Regulatory mechanism:'''
| |
| ** [[Spx]]: transcription activation {{PubMed|23894131}}
| |
|
| |
| * '''Additional information:'''
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:'''
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
| ==Reviews==
| |
| <pubmed> 19883645 16364630 20155482 18523015 </pubmed>
| |
| ==Original publications==
| |
| <pubmed>17179213,12526808,20360175 ,9829937, 16109934 </pubmed>
| |
| ==Operon and expression==
| |
| <pubmed> 20308541 23894131</pubmed>
| |
| [[Category:Protein-coding genes]]
| |
