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| * '''Description:''' [[RNA polymerase]] forespore-specific (early) [[sigma factor]] SigF <br/><br/>
| | {{#bank: SigF}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''sigF''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''spoIIAC ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[RNA polymerase]] forespore-specific <br/>(early) [[sigma factor]] SigF
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || [[transcription]] of sporulation genes (early forespore)
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23450 sigF]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SigF SigF]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 5.078
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 765 bp, 255 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spoVAA]]'', ''[[spoIIAB]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU23450 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU23450 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU23450 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:sigF_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sigF_2443429_2444196_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:sigF_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23450]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[transcription]]}},
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| {{SubtiWiki category|[[sigma factors and their control]]}},
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| {{SubtiWiki category|[[sporulation proteins]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[AbrB regulon]]}},
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| {{SubtiWiki regulon|[[SigF regulon]]}},
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| {{SubtiWiki regulon|[[SigG regulon]]}},
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| {{SubtiWiki regulon|[[SigH regulon]]}},
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| {{SubtiWiki regulon|[[SinR regulon]]}},
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| {{SubtiWiki regulon|[[Spo0A regulon]]}}
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| =The [[SigF regulon]]=
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU23450
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/dacF-spoIIAAB-sigF.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10298]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' sigma-70 factor family (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[SigG]]
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| === Genes controlled by SigF ===
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| ''[[dacF]]-[[spoIIAA]]-[[spoIIAB]]-[[sigF]]'', ''[[sigG]]'','' [[pbpF]]'', ''[[yphA]]-[[seaA]]''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[SigF]]-[[Fin]] (probably) {{PubMed|21037003}}
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| ** [[SigF]]-[[SpoIIAB]]
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| ** [[SigF]]-([[RpoB]]-[[RpoC]])
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| * '''[[Localization]]:''' cell membrane (according to Swiss-Prot)
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1L0O 1L0O] (complex with SpoIIAB, Geobacillus stearothermophilus)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P07860 P07860]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU23450]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| ** ''[[dacF]]-[[spoIIAA]]-[[spoIIAB]]-[[sigF]]''
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| ** ''[[spoIIAA]]-[[spoIIAB]]-[[sigF]]''
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sigF_2443429_2444196_-1 sigF] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| ** ''[[dacF]]'': [[SigF]], [[SigG]]
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| ** ''[[spoIIAA]]'': [[SigH]]
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| * '''Regulation:'''
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| ** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]
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| ** ''[[dacF]]'': expressed during sporulation
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| ** ''[[spoIIAA]]'': expressed early during sporulation
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| ** expressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1556084,15687200 PubMed]
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| * '''Regulatory mechanism:'''
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| ** ''[[spoIIAA]]'': activated by [[Spo0A]] and repressed by [[SinR]]
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| ** [[Spo0A]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/1556084,15687200 PubMed]
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| ** [[AbrB]]: transcription repression {{PubMed|20817675}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| * [[Charles Moran]], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>20833318 </pubmed>
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| ==The [[SigF regulon]]==
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| <pubmed>16497325</pubmed>
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| ==Original Publications==
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| <pubmed>10503549,8358793,7570023,9555886,3007937,8460142,9077448,10323866,10869437,7768847,14744853,8759874,10864495,1744043,16824103,11701124,1902463,8171000,1904527,15351644,15882622,8764398,8764397,9826498,9826499,2492512,1629150,12676949,7592342,11684022,8846916,1948031,8126438, 3114419,12081956,9150232,15205443,9004507,8168129,2123551,8824593,19390092,17498739,8021191,1556084,15687200,12107147, 20298743 18208527 10049355 23169620,21037003,20817675,21935351,22312331</pubmed>
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| [[Category:Protein-coding genes]]
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