Latest revision as of 15:23, 2 April 2014

Description: small RNA

Gene name	bsrF
Synonyms	SR2
Essential	no
Product	small RNA
Function	unknown
Gene length	bp
Immediate neighbours	yobO, csaA
Gene sequence (+200bp)
Genetic context File:GenE context.gif

Categories containing this gene/protein

ncRNA

This gene is a member of the following regulons

The gene

Basic information

Locus tag:

Phenotypes of a mutant

Database entries

BsubCyc: ?

DBTBS entry: no entry

SubtiList entry: no entry

Structure:

Additional information

Expression and regulation

Operon: bsrF PubMed

Sigma factor: SigA PubMed

Regulation: weakly activated by CodY PubMed

Regulatory mechanism: CodY: transcription activation PubMed

Additional information:

Biological materials

Mutants: DB104::Delta bsrF::cat, DB104::Delta bsrF::ery, 168::Delta bsrF::cat, 168::Delta bsrF::ery available in the Sabine Brantl lab

Expression vectors:
- pWSR2, multi-copy vector for tetracycline-inducible expression of bsrF (kanamycin resistance gene), available in the Sabine Brantl lab
- pUCB2SR2, multi-copy vector for constitutive expression of bsrF under its own promoter (kanamycin resistance gene), available in the Sabine Brantl lab

lacZ fusions: pACG1 to pACG19 containing regions of different size upstream of the bsrF promoter available in the Sabine Brantl lab

Labs working on this gene/RNA

Sabine Brantl, Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany homepage
Kouji Nakamura, Tsukuba, University, Japan

Your additional remarks

References

@@ Line 6: / Line 6: @@
 |''bsrF''
 |-
-|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
+|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''SR2 ''
 |-
 |style="background:#ABCDEF;" align="center"| '''Essential''' || no
@@ Line 16: / Line 16: @@
 |style="background:#ABCDEF;" align="center"| '''Gene length''' ||  bp
 |-
-|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' ||
+|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yobO]]'', ''[[csaA]]''
 |-
 |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/genE.txt     Gene sequence      (+200bp)   ]'''
@@ Line 29: / Line 29: @@
 <br/><br/><br/><br/><br/>
+= [[Categories]] containing this gene/protein =
+{{SubtiWiki category|[[ncRNA]]}}
+= This gene is a member of the following [[regulons]] =
 =The gene=
 === Basic information ===
-* '''Coordinates:'''
+* '''Locus tag:'''
 ===Phenotypes of a mutant ===
 === Database entries ===
+* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=?&redirect=T ?]
 * '''DBTBS entry:''' no entry
@@ Line 46: / Line 53: @@
 === Additional information===
 =Expression and regulation=
-* '''Operon:'''
+* '''Operon:''' ''bsrF'' [http://www.ncbi.nlm.nih.gov/sites/entrez/19542274 PubMed]
-* '''Sigma factor:'''
+* '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19542274 PubMed]
-* '''Regulation:'''
+* '''Regulation:''' weakly activated by [[CodY]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19542274 PubMed]
-* '''Regulatory mechanism:'''
+* '''Regulatory mechanism:''' [[CodY]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/19542274 PubMed]
 * '''Additional information:'''
 =Biological materials =
-* '''Mutant:'''
+* '''Mutants:''' DB104::Delta bsrF::cat, DB104::Delta bsrF::ery, 168::Delta bsrF::cat, 168::Delta bsrF::ery available in the  [[Sabine Brantl]] lab
-* '''Expression vector:'''
+* '''Expression vectors:'''
+** pWSR2, multi-copy vector for tetracycline-inducible expression of ''bsrF'' (kanamycin resistance gene), available in the [[Sabine Brantl]] lab
+** pUCB2SR2, multi-copy vector for constitutive expression of ''bsrF'' under its own promoter (kanamycin resistance gene), available in the [[Sabine Brantl]] lab
-* '''lacZ fusion:'''
+* '''lacZ fusions:''' pACG1 to pACG19 containing regions of different size upstream of the ''bsrF'' promoter available in the [[Sabine Brantl]] lab
 =Labs working on this gene/RNA=
-Kouji Nakamura, Tsukuba, University, Japan
+* [[Sabine Brantl |Sabine Brantl]], Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany [http://www.uni-jena.de/AG_Bakteriengenetik_(_Doz__Dr__Sabine_Brantl_).html homepage]
+* Kouji Nakamura, Tsukuba, University, Japan
 =Your additional remarks=
@@ Line 77: / Line 88: @@
 =References=
-# Saito S, Kakeshita H, Nakamura K (2009) Novel small RNA-encoding genes in the intergenic regions of ''Bacillus subtilis''. ''Gene'' '''428:''' 2-8. [http://www.ncbi.nlm.nih.gov/sites/entrez/18948176 PubMed]
+<pubmed>18948176, 19542274 </pubmed>
+[[Category:small RNA]]